Electron-microscopic studies have revealed intracytoplasmic virus particles and unusual intracytoplasmic and intranuclear fibrillar structures in BHK 21 and KB cells infected with Colorado tick fever (CTF) virus. This communication briefly describes these findings.

BHK 21 cells were grown in Eagle’s basal medium (BME) in Hanks’s balanced salt solution (BSS) + 10% foetal bovine serum. Fortified BME in Earle’s BSS containing 10% foetal bovine serum was used as the outgrowth medium for KB cells. The monolayers were grown in 8 oz prescription bottles and were infected with 0.5 ml. of the florio strain of CTF virus (10 TCD 50/ml.) in 10 ml. of maintenance medium. The maintenance medium for BHK 21 cells consisted of BME in Earle’s BSS and 3% foetal bovine serum. Leibovitz 15 (L-15) medium (Leibovitz, 1963) containing 2% inactivated foetal bovine serum was used as the maintenance medium for KB cells.


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