1887

Abstract

Summary

A non-occluded virus of was purified using a chloroform+butanol treatment followed by sucrose gradient centrifugation. Purified virus and top component were produced in proportions of 1:3; their diameters were approximately 200 Å, and their sedimentation coefficients 119 and 58 respectively. The virus had an approximate DNA:protein ratio of 37:63.

Virus and top component were serologically identical in gel diffusion tests and both were different antigenically from insect proteins with sedimentation coefficients of 2 to 3, 17, 30 and 70 to 75, present in both healthy and infected larvae. The amino acid composition of the protein from the virus and top component was very similar, and differed from that of the 17 component. No soluble antigens were detected in the extracts of infected larvae.

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/content/journal/jgv/10.1099/0022-1317-3-2-167
1968-09-01
2022-08-13
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