1887

Abstract

Summary

Freeze-dried preparations of the scrapie agent and of 5 strains of virus were used to determine the extent of protection afforded against ionizing radiation by anoxia. The five viruses tested were bacteriophages T1 and T2 (DNA) and 2 (RNA); and the animal viruses herpes simplex (DNA) and yellow fever (RNA). Oxygen enhancement ratios (measured in terms of doses required to give the same effect in the absence and presence of oxygen) varied from about 1.4 for the scrapie agent to 2.5 for yellow fever virus. With the viruses there was no obvious correlation of oxygen enhancement ratio with type or size of nucleic acid core.

‘Target sizes’ of the viruses were calculated from the doses required to give an average of one lethal event per particle. These agreed well with independent estimates of size of nucleic acid core; except that for bacteriophages T1 and T3 the calculated molecular weight was 0.3 of that determined by Bresler . (1967). Results with the scrapie agent confirmed the previous estimate (Alper, Haig & Clarke, 1966) of a molecular weight of about 1.5 × 10 daltons.

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1968-09-01
2021-10-17
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References

  1. Alexander P. 1957; Effect of oxygen on inactivation of trypsin by the direct action of electrons and alpha particles. Radiat. Res 6:653
    [Google Scholar]
  2. Alper T. 1954; Inactivation of bacteriophage after irradiation. Br. J. Radiol 17:50
    [Google Scholar]
  3. Alper T. 1956; The modification of damage caused by primary ionization of biological targets. Radiat. Res 5:573
    [Google Scholar]
  4. Alper T., Haig D. A., Clarke M. C. 1966; The exceptionally small size of the scrapie agent. Biochem. biophys. Res. Commun 22:278
    [Google Scholar]
  5. Alper T., Cramp W. A., Haig D. A., Clarke M. C. 1967; Does the agent of scrapie replicate without nucleic acid?. Nature, Load 214:764
    [Google Scholar]
  6. Bachofer C. S. 1953; Direct effect of X-rays on bacterial viruses, modified by physical state, in relation to the target theory. Science, N. Y 117:280
    [Google Scholar]
  7. Bachofer C. S., Pottinger M. A. 1954; Oxygen protection against ionizing radiations. Science, N. Y 119:378
    [Google Scholar]
  8. Batchelor A., Bewley D. K., Morrison R., Stevenson J. A. 1959; Electron therapy at 8 Mev. Br. J. Radiol 32:332
    [Google Scholar]
  9. Ben-Porat T., Kaplan A. S. 1962; Chemical composition of herpes simplex and pseudorabies virus. Virology 16:261
    [Google Scholar]
  10. Bergold G. H., Weibel J. 1962; Demonstration of yellow fever virus with the electron microscope. Virology 17:554
    [Google Scholar]
  11. Boag J. W., Dolphin G. W., Rotblat J. 1958; Radiation Dosimetry by transparent plastics. Radial. Res 9:589
    [Google Scholar]
  12. Bresler S. E., Kiselev N. A., Manjakov V. F., Mosevitsky M. I., Tunkovsky A. L. 1967; Isolation and physio-chemical investigation of Tx bacteriophage DNA. Virology 33:1
    [Google Scholar]
  13. Chandler R. L. 1963; Experimental scapie in the mouse. Res. Vet. Sci 4:276
    [Google Scholar]
  14. Dettori R., Maccacaro G. A., Piccinin G. L. 1961; Sex-specific bacteriophages of Escherichia coli K12. G. Microbiol 9:141
    [Google Scholar]
  15. Fluke D. J. 1966; Dry T1 bacteriophage in measurement of large doses of ionizing radiation. Radial. Res 28:336
    [Google Scholar]
  16. Ginoza W. 1967; The effects of ionizing radiation on nucleic acids of bacteriophages and bacterial cells. A. Rev. Microbiol 21:325
    [Google Scholar]
  17. Henley E. J., Richman D. 1956; Cellophane dye-dosimeter for 105 to 107 R-range. Analyt. Chem 28:1580
    [Google Scholar]
  18. Hewitt H. B., Read J. 1950; Search for an effect of oxygen on the direct X-ray inactivation of bacteriophage. Br. J. Radiol 23:416
    [Google Scholar]
  19. Hill R. F. 1958; A radiation sensitive mutant of Escherichia coli . Biochim. biophys. Acta 30:636
    [Google Scholar]
  20. Howard-Flanders P. 1961; Factors affecting radiation injury to DNA in bacteria and bacteriophage systems. Brookhaven Symposia in Biology 14:18
    [Google Scholar]
  21. Hunt J. W., Till J. E., Williams J. F. 1962; Radiation damage and free radical production in irradiated ribonuclease. Radial. Res 17:703
    [Google Scholar]
  22. Lea D. E. 1946 Actions of Radiations on Living Cells chap. 3 Cambridge University Press;
    [Google Scholar]
  23. Mitra S., Enger M. D., Kaesberg P. 1963; Physical and chemical properties of RNA from the bacterial virus R17. Proc. natn. Acad. Sci. U.S.A. 50:68
    [Google Scholar]
  24. Pike M. C., Alper T. 1964; A method for determining dose-modification factors. Br. J. Radiol 37:458
    [Google Scholar]
  25. Russell W. C., Crawford L. V. 1963; Some characteristics of the deoxyribonucleic acid from herpes simplex virus. Virology 21:353
    [Google Scholar]
  26. Russell W. C., Crawford L. V. 1964; Properties of the nucleic acid from herpes group of viruses. Virology 22:288
    [Google Scholar]
  27. Schambra P. E., Hutchinson F. 1964; The action of fast heavy ions on biological material. II. Effects on Tx and ϕ X–174 bacteriophage and double-strand and single-strand DNA. Radial. Res 23:514
    [Google Scholar]
  28. Shalek R. I., Gillespie T. L. 1960; The influence of oxygen upon the radiation damage of lysozyme. In Radiation Biology and Cancer p. 41 London: Peter Owen Ltd;
    [Google Scholar]
  29. Tanooka H., Hutchinson F. 1965; Modifications of the inactivation by ionizing radiations of the transforming activity of DNA in spores and dry cells. Radial. Res 24:43
    [Google Scholar]
  30. Zinder N. D. 1955; RNA Phages. Ann. Rev. Microbiol 19:455
    [Google Scholar]
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