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Volume 3, Issue 1

The Morphological and Biological Effects of Various Antisera on Avian Infectious Bronchitis Virus Free

Abstract

Summary

Biologically, homotypic and heterotypic antisera neutralized avian infectious bronchitis virus significantly more when unheated. Morphologically, using the electron microscope technique of negative staining, there was a clear distinction between the effects of homotypic and heterotypic antisera. Heated homotypic antiserum revealed antibody attached only to the projections of the virus, while with unheated homotypic serum heat labile components could be visualized but no basic change could be seen in particle morphology. Heterotypic serum contained antibodies directed both against the projections and the envelope of the virus. In addition, unheated heterotypic antiserum produced holes approximately 100 Å in diameter in the virus membrane, suggesting that a form of virus lysis takes place. Rabbit antiserum prepared against uninfected chick-embryo fibroblasts was able to produce similar holes in the virus envelope and this led us to postulate that the envelope component of avian infectious bronchitis virus is closely related to normal chick host material.

  • Received:
  • Accepted:
  • Published Online:
© Journal of General Virology 1968
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1968-07-01
2024-04-19
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References

  1. Almeida J., Cinader B., Howatson A. 1963; The structure of antigen-antibody complexes. J. exp. Med 118:327
     [Google Scholar]
  2. Benson H. N., Brumfield H. P., Pomeroy B. S. 1961; Requirement of avian C′ i for fixation of guinea pig complement by avian antibody antigen complexes. J. Immun 87:616
     [Google Scholar]
  3. Berry D. M., Cruickshank J. G., Chu H. P., Wells R. J. H. 1964; The structure of infectious bronchitis virus. Virology 23:403
     [Google Scholar]
  4. Borsos T., Dourmashkin R. R., Humphrey J. H. 1964; Lesions in erythrocyte membranes caused by immune haemolysis. Nature, Lond 202:251
     [Google Scholar]
  5. Cunningham C. H. 1951; Newcastle disease and infectious bronchitis neutralizing antibody indices of normal chicken serum. Am. J. vet. Res 12:129
     [Google Scholar]
  6. Feinstein A., Munn E. A. 1966; An electron microscopic study of the interaction of macroglobulin (IgM) antibodies with bacterial flagella and of the binding of complement. J. Physiol 186:64
     [Google Scholar]
  7. Hummeler K., Anderson T. F., Brown R. A. 1962; Identification of poliovirus particles of different antigenicity by specific agglutination as seen in the electron microscope. Virology 16:84
     [Google Scholar]
  8. Humphrey J. H., Dourmashkin R. R. 1965; Ciba Symposium on Complement. p 175
     [Google Scholar]
  9. Lafferty K. J., Oertelis S. J. 1961; Attachment of antibody of influenza virus. Nature, Lond 192:764
     [Google Scholar]
  10. Lennette E. H. 1964 In Diagnostic procedures for viral and rickettsial diseases. N. Y. Am. Publ. Hlth. Assoc. Inc 1964, Ch. 1, p 43
     [Google Scholar]
  11. Page C. A., Cunningham C. H. 1962; The neutralization test for infectious bronchitis virus. Am. J. vet. Res 23:1065
     [Google Scholar]
  12. Yoshino K., Taniguchi S. 1965; Studies on the neutralization of herpes simplex virus 1, 11, in. Virology 26:44, 54, 61
     [Google Scholar]
  13. Yoshino K., Taniguchi S. 1967; Studies on the neutralization of herpes simplex virus. Virology 31:260
     [Google Scholar]
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The Morphological and Biological Effects of Various Antisera on Avian Infectious Bronchitis Virus
J. Gen. Virol. 3, 97 (1968); https://doi.org/10.1099/0022-1317-3-1-97
/content/journal/jgv/10.1099/0022-1317-3-1-97
/content/journal/jgv/10.1099/0022-1317-3-1-97
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