1887

Abstract

As part of a study of the way in which tobacco rattle viruses multiply, we have examined the intracellular distribution of the isolate (Harrison & Woods, 1966) by means of electron microscopy of thin sections of leaves. The micrographs show a remarkable association between virus particles and mitochondria. Nothing comparable seems to have been reported with other viruses.

Tip leaves, about 2 cm. long, were taken from plants of Gray 5 days after their lower leaves were inoculated with the isolate. Narrow strips of leaf blade were fixed in 5% (v/v) glutaraldehyde, post-fixed in 0.1% OO, stained during dehydration for 16 hr in a saturated solution of uranyl acetate in 70% (v/v) acetone, and embedded in methyl methacrylate + styrene (6:1, v/v). Sections, mostly about 600 Å thick, were cut with a Huxley ultramicrotome, using a glass knife.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-3-1-121
1968-07-01
2022-01-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/3/1/JV0030010121.html?itemId=/content/journal/jgv/10.1099/0022-1317-3-1-121&mimeType=html&fmt=ahah

References

  1. Harrison B. D., Woods R. D. 1966; Serotypes and particle dimensions of tobacco rattle viruses from Europe and America. Virology 28:610
    [Google Scholar]
  2. Lister R. M. 1966; Possible relationships of virus-specific products of tobacco rattle virus infections. Virology 28:350
    [Google Scholar]
  3. Frost R. R., Harrison B. D., Woods R. D. 1967; Apparent symbiotic interaction between particles of tobacco rattle virus. J. gen. Virol 1:57
    [Google Scholar]
  4. Cadman C. H. 1962; Evidence for association of tobacco rattle virus nucleic acid with a cell component. Nature, Land 193:49
    [Google Scholar]
  5. de Zoeten G. A. 1966; California tobacco rattle virus, its intracellular appearance, and the cytology of the infected cell. Phytopathology 56:744
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-3-1-121
Loading
/content/journal/jgv/10.1099/0022-1317-3-1-121
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error