Biophysical properties of Mycobacteriophages Free

Abstract

SUMMARY

Biophysical characterization of two mycobacteriophages (Phlei phage and Butyricum phage) was carried out. Biophysical parameters obtained were: (i) buoyant densities in CsCl of 1.51 g/ml for both phages; (ii) sedimentation coefficient of 490 S and 410S; (iii) DNA content of 42 and 34% and (iv) mol. wt. calculated by electron microscopic dimensions to be 123 × 10 and 116 × 10 for Butyricum and Phlei phage, respectively.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-29-2-235
1975-11-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/29/2/JV0290020235.html?itemId=/content/journal/jgv/10.1099/0022-1317-29-2-235&mimeType=html&fmt=ahah

References

  1. Adams M. H. 1959 In Bacteriophages pp 450–451 New York: Interscience Publishers Inc;
    [Google Scholar]
  2. Albertsson P. A. 1967; Two phase separation of viruses. In Methods in Virology vol 2 pp 303–321 Edited by Maramorosch K., Koprowski H. New York and London: Academic Press;
    [Google Scholar]
  3. Bradley D. E. 1963; The structure of coliphages. Journal of General Virology 31:435–445
    [Google Scholar]
  4. Bradley D. E. 1967; Ultrastructure of bacteriophages and bacteriocins. Bacteriological Reviews 31:230–314
    [Google Scholar]
  5. Castelnuovo G. 1970 In Host-virus Relationships in Mycobacterium nocardia andactinomyces Chapter 2 pp 15–28 Edited by Juhasz S. E., Plummer G. Springfield, Illinois: C. C. Thomas;
    [Google Scholar]
  6. Castelnuovo G., Bellezza G., Borelli F. 1970; Les antigenes d’un mycobacteriophages: phage Phlei. Annales de I’lnstitut Pasteur, Paris 118:214–219
    [Google Scholar]
  7. Castelnuovo G., Giuliani H. I., Luchini De-giuliani E. 1969; Protein components of a mycobacterio-phage. Journal of General Virology 4:253–257
    [Google Scholar]
  8. Harrison D. P., Brown D. T., Bode V. C. 1973; The lambda head-tail joining reaction: purification, properties and structure of biologically active heads and tails. Journal of Molecular Biology 79:437–449
    [Google Scholar]
  9. Hosaka Y. 1965; A criterion for evaluating the number of capsomeres of icosahedral capsids. Biochimica et Biophysica Acta 104:261–273
    [Google Scholar]
  10. Hubbard R. W., Matthew W. T., Dubowik D. A. 1970; Factors influencing the determination of DNA with indole. Analytical Biochemistry 38:190–201
    [Google Scholar]
  11. Kölbel H. 1970 In Host-Virus Relationships in Mycobacterium nocardia and actinomyces Chapter 3, pp 29–38 Edited by Juhasz S. E., Plummer G. Springfield, Illinois: C. C. Thomas;
    [Google Scholar]
  12. Nermut M. V., Frank H., Schäfer W. 1972; Properties of mouse leukemia viruses. III. Electromicroscopic appearance as revealed after conventional preparation techniques as well as freeze-drying and freeze-etching. Virology 49:345–358
    [Google Scholar]
  13. Redmond W. B. 1963; Bacteriophages of the mycobacteria. Advances in Tuberculosis Research 12:191–229
    [Google Scholar]
  14. Sellers H. I., Tokunaga T. 1966; Further studies of infectious DNA extracted from mycobacteriophages. Journal of Experimental Medicine 123:327–340
    [Google Scholar]
  15. Takeya K., Amako K. 1964; The structure of mycobacteriophages. Virology 24:461–466
    [Google Scholar]
  16. Tikchonenko T. I., Koudelka J., Borishpolez Z. I. 1963; Concentration and purification of phages by the method of column chromatography. Mikrobiologiya 32:723–726
    [Google Scholar]
  17. Tokunaga T., Sellers M. I. 1964; Infection of Mycobacterium smegmatis with D29 phage DNA. Journal of Experimental Medicine 119:139–149
    [Google Scholar]
  18. Vandra E. 1965 Thesis. Hungarian Academy of Sciences; Budapest:
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-29-2-235
Loading
/content/journal/jgv/10.1099/0022-1317-29-2-235
Loading

Data & Media loading...

Most cited Most Cited RSS feed