1887

Abstract

SUMMARY

Poliovirus proteins were labelled with [S]-methionine, and the major products of translation and cleavage were separated by electrophoresis and compared in terms of two-dimensional tryptic peptide maps visualized by autoradiography. The main intermediates p110 and p90 had few or no methionine-labelled sequences in common, but were both contained in, and therefore almost fully account for, the presumed primary translation product p210. The sequences of p79, a major stable product of cleavage and a non-structural protein, were almost completely contained in p90, which in turn is the major component of the larger intermediates p168 and p155. P110 is confirmed as the precursor of virus particle protein, and VPo as the precursor of VP2. However, the sequences of p31, the other major product of translation that is not a structural protein, were not contained in any of the viral polypeptides mentioned above.

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/content/journal/jgv/10.1099/0022-1317-29-2-215
1975-11-01
2024-05-25
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References

  1. Abraham G., Cooper P. D. 1975a; Poliovirus polypeptides examined in more detail. (preceding paper)
    [Google Scholar]
  2. Abraham G., Cooper P. D. 1975b; Anomalous behavior of certain poliovirus polypeptides during SDS-gel electrophoresis. (in the press)
    [Google Scholar]
  3. Cole C. N., Baltimore D. 1973; Defective interfering particles of poliovirus II Nature of the defect. Journal of Molecular Biology 76:325–343
    [Google Scholar]
  4. Cooper P. D., Bennett D. J. 1973; Genetic and structural implications of tryptic peptide analysis of poliovirus structural protein. Journal of General Virology 20:151–160
    [Google Scholar]
  5. Jacobson M. F., Asso J., Baltimore D. 1970; Further evidence on the formation of poliovirus proteins. Journal of Molecular Biology 49:657–669
    [Google Scholar]
  6. Laver W. G. 1969; Peptide mapping of viral proteins. In Fundamental Techniques in Virology pp 371–378 Edited by Habel K., Salzman N. P. New York and London: Academic Press;
    [Google Scholar]
  7. Lundquist R. E., Ehrenfeld E. E., Maizel J. V. 1974; Isolation of a viral polypeptide associated with poliovirus RNA polymerase. Proceedings of the National Academy of Sciences of the United States of America 71:4773–4777
    [Google Scholar]
  8. Shaw E. N. 1967; Site-specific reagents for chymotrypsin and trypsin. In Methods in Enzymology vol xi pp 677–688 Edited by Colowick S. P., Kaplan N. O. New York: Academic Press;
    [Google Scholar]
  9. Summers D. F., Maizel J. V. 1971; Determination of the gene sequence of poliovirus with pactamycin. Proceedings of the National Academy of Sciences of the United States of America 68:2852–2856
    [Google Scholar]
  10. Summers D. F., Roumiantzeff M., Maizel J. 1971; The translation and processing of poliovirus proteins. In Strategy of the Viral Genome pp 111–133 Edited by Wolstenholme G. E. W., O’Connor M. London: Churchill Livingstone;
    [Google Scholar]
  11. Taber R., Rekosh D., Baltimore D. 1971; Effect of pactamycin on synthesis of poliovirus proteins: a method for genetic mapping. Journal of Virology 8:395–401
    [Google Scholar]
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