1887

Abstract

SUMMARY

The multiplication of a temperature-sensitive () mutant of cowpea chlorotic mottle virus (CCMV) in tobacco protoplasts has been examined. The results were similar to those obtained with intact cowpea plants. No intact virus was produced above 33.5 °C, and no coat protein, either soluble or insoluble was detected in the absence of intact virus. Ribonucleic acid (RNA) of the mutant, however, was synthesized at 35 °C. Virus particles made at 25 °C were degraded when the infected protoplasts were subsequently cultured at 35 °C. RNA-3 of CCMV was apparently encapsidated much more often than RNA-3 of wild-type () CCMV in doubly infected protoplasts cultured at either 25 or 35 °C.

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/content/journal/jgv/10.1099/0022-1317-29-1-99
1975-10-01
2024-04-12
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References

  1. Atabekov J. G., Schaskolskaya N. D., Atabekova T. I., Sacharovskaya G. A. 1970; Reproduction of temperature-sensitive strains of TMV under restrictive conditions in the presence of temperature-resistant helper strain. Virology 41:397–407
    [Google Scholar]
  2. Bancroft J. B., Lane L. C. 1973; Genetic analysis of cowpea chlorotic mottle and brome mosaic virus. Journal of General Virology 19:381–389
    [Google Scholar]
  3. Bancroft J. B., Motoyoshi F., Watts J. W., Dawson J. R. O. 1975; Cowpea chlorotic mottle and brome mosaic viruses in tobacco protoplasts. John Innes Institute Symposium 1974: PP 349
    [Google Scholar]
  4. Bancroft J. B., Rees M. W., Dawson J. R. O., Mclean G. D., Short M. N. 1972; Some properties of a temperature-sensitive mutant of cowpea chlorotic mottle virus. Journal of General Virology 16:69–81
    [Google Scholar]
  5. Goodman D., Matzura H. 1971; An improved method of counting radioactive acrylamide gels. Analytical Biochemistry 42:481–486
    [Google Scholar]
  6. Hariharasubramanian V., Zaitlin M., Siegel A. 1970; A temperature-sensitive mutant of TMV with unstable coat protein. Virology 40:579–589
    [Google Scholar]
  7. Jockusch H. 1968; Two mutants of tobacco mosaic virus temperature-sensitive in two different functions. Virology 35:94–101
    [Google Scholar]
  8. Kassanis B., Bastow C. 1971; Phenotypic mixing between strains of tobacco mosaic virus. Journal of General Virology 11:171–176
    [Google Scholar]
  9. Loening U. E. 1967; The fractionation of high-molecular-weight ribonucleic acid by polyacrylamide-gel electrophoresis. Biochemical Journal 102:251–257
    [Google Scholar]
  10. Motoyoshi F., Bancroft J. B., Watts J. W., Burgess J. 1973a; The infection of tobacco protoplasts with cowpea chlorotic mottle virus and its RNA. Journal of General Virology 20:177–193
    [Google Scholar]
  11. Motoyoshi F., Bancroft J. B., Watts J. W. 1973b; A direct estimate of the number of cowpea chlorotic mottle virus particles absorbed by tobacco protoplasts that become infected. Journal of General Virology 21:159–161
    [Google Scholar]
  12. Sarkar S. 1969; Evidence of phenotypic mixing between two strains of tobacco mosaic virus. Molecular and General Genetics 105:87–90
    [Google Scholar]
  13. Swank R. T., Munkres K. D. 1971; Molecular weight analysis of oligopeptides by electrophoresis in polyacrylamide gel with sodium dodecyl sulphate. Analytical Biochemistry 39:462–477
    [Google Scholar]
  14. Van Loon L. C., Van Kammen A. 1968; Polyacrylamide disc electrophoresis of the soluble leaf proteins from Nicotiana tabacum var. ‘Samsun’ and ‘Samsun NN’. 1. Phytochemistry 7:1727–1735
    [Google Scholar]
  15. Weber K., Osborn M. 1969; The reliability of molecular weight determinations by dodecyl sulphate-polyacrylamide gel electrophoresis. Journal of Biological Chemistry 244:4406–4412
    [Google Scholar]
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