1887

Abstract

SUMMARY

A procedure was developed to isolate tomato mesophyll protoplasts in amounts sufficient for studying virus infection and multiplication. The infection of protoplasts with a tomato strain of tobacco mosaic virus occurred most efficiently when the inoculum contained 1 µg/ml virus, 1 µg/ml poly--ornithine and 0.01 -phosphate buffer, pH 6.7. The efficiency of infection was influenced by the concentration of ions as well as by the pH of inoculation buffer. The virus infected protoplasts of plants homozygous for the gene Tm-2, which is responsible for TMV-resistance of intact leaves, and multiplied as rapidly in them as in protoplasts from the susceptible parent line. In TMV-inoculated leaf discs prepared from the resistant plants, however, the growth of virus was very limited when compared with the large yield of virus obtained from leaf discs of the susceptible plants. The possible nature of the resistance is discussed in the light of these findings.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-29-1-81
1975-10-01
2024-12-08
Loading full text...

Full text loading...

/deliver/fulltext/jgv/29/1/JV0290010081.html?itemId=/content/journal/jgv/10.1099/0022-1317-29-1-81&mimeType=html&fmt=ahah

References

  1. Alexander L. J. 1971; Host-pathogen dynamics of tobacco mosaic virus on tomato. Phytopathology 61:611–617
    [Google Scholar]
  2. Aoki S., Takebe I. 1969; Infection of tobacco mesophyll protoplasts by tobacco mosaic virus ribonucleic acid. Virology 39:439–448
    [Google Scholar]
  3. Aoki S., Takebe I. 1975; Replication of tobacco mosaic virus RNA in tobacco mesophyll protoplasts inoculated in vitro. Virology 65:343–354
    [Google Scholar]
  4. Cirulli M., Alexander L. L. 1969; Influence of temperature and strain of tobacco mosaic virus on resistance in a tomato breeding line derived from Lycopersicon peruvianum. Phytopathology 59:1287–1297
    [Google Scholar]
  5. Cocking E. C., Fojnar E. 1969; An electron microscopic study of the infection of isolated tomato fruit protoplasts by tobacco mosaic virus. Journal of General Virology 4:305–312
    [Google Scholar]
  6. Fraenkel-Conrat H. 1966; Preparation and testing of tobacco mosaic virus-RNA. In Procedure in Nucleic Acid Research pp 480–487 Edited by Cantoni G. L., Davies D. R. New York and London: Harper & Row;
    [Google Scholar]
  7. Hibi T., Yora K. 1972; Electron microscopy of tobacco mosaic virus infection in tobacco mesophyll protoplasts. Annals of the Phytopathological Society of Japan 38:350–356
    [Google Scholar]
  8. Kubo S., Harrison B. D., Robinson D. J. 1974; Effect of phosphate on the infection of tobacco protoplasts by tobacco rattle virus. Intervirology 3:382–387
    [Google Scholar]
  9. Motoyoshi F., Bancroft J. B., Watts J. W. 1974a; The infection of tobacco protoplasts with a variant of brome mosaic virus. Journal of General Virology 25:31–36
    [Google Scholar]
  10. Motoyoshi F., Bancroft J. B., Watts J. W., Burgess J. 1973; The infection of tobacco protoplasts with cowpea chlorotic mottle virus and its RNA. Journal of General Virology 20:177–193
    [Google Scholar]
  11. Motoyoshi F., Hull R. 1974; The infection of tobacco protoplasts with pea enation mosaic virus. Journal of General Virology 24:89–99
    [Google Scholar]
  12. Motoyoshi F., Watts J. W., Bancroft J. B. 1974b; Factors influencing the infection of tobacco protoplasts by cowpea chlorotic mottle virus. Journal of General Virology 25:245–246
    [Google Scholar]
  13. Oshima N., Goto T., Sato R. 1964; A strain of tobacco mosaic virus (TMV-L) isolated from tomato. Research Bulletin No. 83 of the Hokkaido National Agricultural Experimental Station87–99
    [Google Scholar]
  14. Oshima N., Ohashi Y., Umekawa M. 1971; Studies of some strains of tobacco mosaic virus pathogenic to crucifer plants, 1. Physical and chemical studies. Annals of the Phytopathological Society of Japan 37:319–325
    [Google Scholar]
  15. Otsuki Y., Shimomura T., Takebe I. 1972; Tobacco mosaic virus multiplication and expression of the N gene in necrotic responding tobacco varieties. Virology 50:45–50
    [Google Scholar]
  16. Otsuki Y., Takebe I. 1969a; Fluorescent antibody staining of tobacco mosaic virus antigen in tobacco mesophyll protoplasts. Virology 38:497–499
    [Google Scholar]
  17. Otsuki Y., Takebe I. 1969b; Isolation of intact mesophyll cells and their protoplasts from higher plants. Plant and Cell Physiology 10:917–921
    [Google Scholar]
  18. Otsuki Y., Takebe I. 1973; Infection of tobacco mesophyll protoplasts by cucumber mosaic virus. Virology 53:433–438
    [Google Scholar]
  19. Otsuki Y., Takebe I., Honda Y., Kajita S., Matsui C. 1974; Infection of tobacco mesophyll protoplasts by potato virus X. Journal of General Virology 22:375–385
    [Google Scholar]
  20. Pelham J. 1966; Resistance in tomato to tobacco mosaic virus. Euphytica 15:258–267
    [Google Scholar]
  21. Pelham J. 1972; Strain-genotype interaction of tobacco mosaic virus in tomato. Annals of Applied Biology 71:219–228
    [Google Scholar]
  22. Sakai F., Takebe I. 1974; Protein synthesis in tobacco mesophyll protoplasts induced by tobacco mosaic virus infection. Virology 62:426–433
    [Google Scholar]
  23. Takebe L. 1975; The use of protoplasts in plant virology. Annual Review of Phytopathology (in the press)
    [Google Scholar]
  24. Takebe L., Otsuki Y. 1969; Infection of tobacco mesophyll protoplasts by tobacco mosaic virus. Proceedings of the National Academy of Sciences of the United States of America 64:843–848
    [Google Scholar]
  25. Takebe L., Otsuki Y., Aoki S. 1968; Isolation of tobacco mesophyll cells in intact and active state. Plant and Cell Physiology 9:115–124
    [Google Scholar]
  26. Wakasa K. 1973; Isolation of protoplasts from various plant organs. Japanese Journal of Genetics 48:279–289
    [Google Scholar]
  27. Watts J. W., King J. M. 1973; A method for the preparation of plant protoplasts. Zeitschrift für Naturforschung 28:231
    [Google Scholar]
  28. Watts J. W., Motoyoshi F., King J. M. 1974; Problems associated with the production of stable protoplasts of cells of tobacco mesophyll. Annals of Botany 38:667–671
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-29-1-81
Loading
/content/journal/jgv/10.1099/0022-1317-29-1-81
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error