An RNA-synthesizing complex was found in the nucleoplasm of fowl plague virus-infected chicken fibroblast and Ehrlich tumour cells. The complex sedimented at 120 S and banded in caesium chloride at 1.39 to 1.41 g/ml. It contained an influenza nucleocapsid protein as a major protein constituent. The complex functioned late in infection, and RNA synthesis in it was resistant to actinomycin D, the properties expected of influenza virus replicative complex.
LebedevaE. N.,
VladimirtzevaE. A.,
BakunzK. A.,
BykovskyA. P. H.,
BukrinskayaA. G.1974; Ribo-nucleoprotein of Sendai virus in infected cell nucleolus and its function. Voprosy Virusotogii (Russia) 3:292–298
PenmanS.1969; Preparation of purified nuclei and nucleoli from mammalian cells. In Fundamental Techniques in Virology pp 35–48 New York: Academic Press Inc;
PonsM. W.1971; Isolation of influenza virus ribonucleoprotein from infected cells. Demonstration of the presence of negative-stranded RNA in viral RNP. Virology 46:146–160
ScholtissekC.,
RottR.1970; Synthesis in vitro of influenza virus plus and minus strand RNA and its preferential inhibition by antibiotics. Virology 40:989–996