1887

Abstract

SUMMARY

Rous-associated virus-61 was used as a model for studying the labelling specificity of the chloramine-T iodination procedure when applied to intact enveloped viruses. The specificity of labelling depended markedly on the concentration of iodide in the reaction mixture. At low concentrations of iodide (below 0.5 µ) only the surface proteins and lipid of intact virions were iodinated; there was no detectable labelling of internal proteins. At 10 µ-iodide, however, both internal and external proteins were iodinated; moreover there was a marked change in the reactivity of the surface proteins. It appears that the lipid envelope provides an effective barrier to the iodinating complex generated at low, but not at high, concentrations of iodide. These and other observations suggest that the chloramine-T procedure has a previously unrecognized potential for specifically labelling the surface proteins of lipid-enveloped structures.

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1975-10-01
2022-11-27
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References

  1. August J. T., Bolognesi D. B., Fleissner E., Gilden R. V., Nowinski R. C. 1974; A proposed nomenclature for the virions of oncogenic RNA viruses. Journal of Virology 60:595–601
    [Google Scholar]
  2. Davis N. L., Rueckert R. R. 1972; Properties of a ribonucleoprotein particle isolated from Nonidet P-40-treated Rous sarcoma virus. Journal of Virology 10:1010–1020
    [Google Scholar]
  3. Greenwood F. C., Hunter W. M. 1963; The preparation of 131I-labelled human growth hormones of high specific radioactivity. Biochemical Journal 89:114–123
    [Google Scholar]
  4. Hallaba E., Drouet J. 1971; 131I-labelling of human serum albumin by the iodine monochloride and chloramine-T methods. International Journal of Applied Radiation and Isotopes 22:46–49
    [Google Scholar]
  5. Hunter W. M. 1966 In Radioactive Pharmaceuticals pp 245–264 Edited by Andrews G. A., Kinseley R. M., Wagner H. N. Oak Ridge: U. S. Atomic Energy Commission, Division of Technical Information;
    [Google Scholar]
  6. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  7. Medappa K. C., Mclean C., Rueckert R. R. 1971; On the structure of rhinovirus 1 A. Virology 44:259–270
    [Google Scholar]
  8. Montelaro R. C., Rueckert R. R. 1975; Radiolabeling of proteins and viruses in vitro by acetylation with radioactive acetic anhydride. Journal of Biological Chemistry 250:1413–1421
    [Google Scholar]
  9. Moore N. F., Kelley J. M., Wagner R. R. 1974; Envelope proteins of vesicular stomatitis virus: accessibility to iodination. Virology 61:292–296
    [Google Scholar]
  10. Phillips D. R., Morrison M. 1971; Exposed protein on the intact human erythrocyte. Biochemistry 10:1766–1771
    [Google Scholar]
  11. Poláková K., Russ G. 1974; Localization of major group-specific polypeptides in the avian RNA tumor viruses. Biochemical and Biophysical Research Communications 57:1130–1136
    [Google Scholar]
  12. Stanley P., Haslam E. A. 1971; The polypeptides of influenza virus. V. Localization of polypeptides in the virion by iodination techniques. Virology 46:764–773
    [Google Scholar]
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