1887

Abstract

SUMMARY

Eighteen new mutants of poliovirus have been isolated after a variety of mutagenic treatments, and their loci identified in relation to the previous genetic map. The map was only extended by 25%, and the physiological characters of the new isolates corresponded in all aspects tested with those of the previous isolates. Apparently single mutants at the extreme left of the map were defective in synthesis of both double- and single-stranded RNA, functions that do not co-vary in other mutants. Two procedures respectively predicted to induce mutations preferentially in the 5′ and 3′ regions of the genome gave isolates which all indicated that the structural protein region was nearest the 5′ end. The loci for resistance to dextran sulphate and to ethyl-2-methylthio-4-methyl-5-pyrimidine carboxylate both lie in the structural protein region.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-29-1-109
1975-10-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/jgv/29/1/JV0290010109.html?itemId=/content/journal/jgv/10.1099/0022-1317-29-1-109&mimeType=html&fmt=ahah

References

  1. Béchet J.-M. 1972a; Isolation of the minus strand of encephalomyocarditis virus RNA. Virology 48:855–857
    [Google Scholar]
  2. Béchet J.-M. 1972b; Isolement de la chaine ‘moins’ d’acide ribonucleique de l’encephalomyocardité de la souris et etude de son infectivite. Compte rendu hebdomadaire des seances de VAcademie des Sciences, Paris D 274:1761–1764
    [Google Scholar]
  3. Bengtsson S. 1965; Mechanism of dextran sulphate inhibition of attenuated poliovirus. Proceedings of the Society of Experimental Biology and Medicine 118:47–53
    [Google Scholar]
  4. Bengtsson S. 1966; Counter-current distribution of poliovirus type 2 and 3. Acta Pathologica et Microbiologica Scandinavica 68:68–80
    [Google Scholar]
  5. Bengtsson S. 1968; Attempts to map the poliovirus genome by analysis of selected recombinants. Acta Pathologica et Microbiologica Scandinavica 73:592–604
    [Google Scholar]
  6. Bengtsson S., Philipson L. 1963; Countercurrent distribution of poliovirus type 1. Virology 20:176–184
    [Google Scholar]
  7. Bengtsson S., Philipson L., Persson H., Laurent T. C. 1964; The basis for the interaction between attenuated poliovirus and polyions. Virology 24:617–625
    [Google Scholar]
  8. Best M., Evans B., Bishop J. M. 1972; Double-stranded replication form of poliovirus RNA: phenotype of heterozygous molecules. Virology 47:592–603
    [Google Scholar]
  9. Cooper P. D. 1964; The mutation of poliovirus by 5-fiuorouracil. Virology 22:186–192
    [Google Scholar]
  10. Cooper P. D. 1968; A genetic map of poliovirus temperature-sensitive mutants. Virology 35:584–596
    [Google Scholar]
  11. Cooper P. D. 1969; The genetic analysis of poliovirus. In Biochemistry of Viruses pp 177–218 Edited by Levy H. B. New York: Marcel Dekker;
    [Google Scholar]
  12. Cooper P. D., Bennett D. J. 1973; Genetic and structural implications of tryptic peptide analysis of poliovirus structural protein. Journal of General Virology 20:151–160
    [Google Scholar]
  13. Cooper P. D., Geissler E., Scotti P. D., Tannock G. A. 1971; Further characterisation of the genetic map of poliovirus temperature-sensitive mutants. In Strategy of the Viral Genome, Ciba Foundation Symposium pp 75–100 Edited by Wolstenholme G. E. W., O’Connor Maeve. London: Churchill Livingstone;
    [Google Scholar]
  14. Cooper P. D., Johnson R. T., Garwes D. J. 1966; Physiological characterization of heat-defective (temperature-sensitive) poliovirus mutants: preliminary classification. Virology 30:638–649
    [Google Scholar]
  15. Cooper P. D., Stancek D., Summers D. F. 1970a; Synthesis of double-stranded RNA by poliovirus temperature-sensitive mutants. Virology 40:971–977
    [Google Scholar]
  16. Cooper P. D., Steiner-Pryor A., Scotti P. D., Delong D. 1974; On the nature of poliovirus genetic recombinants. Journal of General Virology 23:41–49
    [Google Scholar]
  17. Cooper P. D., Summers D. F., Maizel J. V. 1970b; Evidence for ambiguity in the post translational cleavage of poliovirus protein. Virology 41:408–418
    [Google Scholar]
  18. Cooper P. D., Wentworth B. B., Mccahon D. 1970c; Guanidine inhibition of poliovirus: a dependence of virus RNA synthesis on the configuration of structural protein. Virology 40:486–493
    [Google Scholar]
  19. Ehrenfeld E., Maizel J. V., Summers D. F. 1970; Soluble RNA polymerase complex from poliovirus-infected HeLa cells. Virology 40:840–846
    [Google Scholar]
  20. GarWes D. J., Wright P. J., Cooper P. D. 1975; Poliovirus temperature-sensitive mutants defective in cytopathic effects are also defective in synthesis of double-stranded RNA. Journal of General Virology 27:45–60
    [Google Scholar]
  21. Koch G., Quintrell N., Bishop J. M. 1967; Differential effect of actinomycin D on the infectivity of single- and double-stranded poliovirus RNA. Virology 31:388–390
    [Google Scholar]
  22. Mccahon D., Cooper P. D. 1970; Identification of poliovirus temperature-sensitive mutants having defects in virus structural protein. Journal of General Virology 6:51–62
    [Google Scholar]
  23. öberg B., Philipson L. 1971; Replicative structures of poliovirus RNA in vivo. Journal of Molecular Biology 58:725–737
    [Google Scholar]
  24. Pagano J. S. 1965; Equine and bovine serums and dextran sulphate as genetic markers of poliovirus. Annals of the New York Academy of Sciences 130:398–403
    [Google Scholar]
  25. Perez-Bercoff R., Cioe L., Meo P., Carrara G., Mechali M., Falcoff E., Rita G. 1974; Infectivity of mengovirus replicative form. Relationship to cellular transcription. Journal of General Virology 25:53–62
    [Google Scholar]
  26. Roy P., Bishop D. H. L. 1970; Isolation and properties of poliovirus minus strand ribonucleic acid. Journal of Virology 6:604–609
    [Google Scholar]
  27. Scharff M. D., Summers D. F., Levintow L. 1965; Further studies on the effect of p-fluorophenylalanine and puromycin on poliovirus replication. Annals of the New York Academy of Sciences 130:282–290
    [Google Scholar]
  28. Spector D. H., Baltimore D. 1974; Requirement of 3′-terminal poly (adenylic acid) for the infectivity of poliovirus RNA. Proceedings of the National Academy of Sciences of the United States of America 71:2983–2987
    [Google Scholar]
  29. Summers D. F., Maizel J. V. 1971; Determination of the gene sequence of poliovirus with pactamycin. Proceedings of the National Academy of Sciences of the United States of America 68:2852–2856
    [Google Scholar]
  30. Taber R., Rekosh D., Baltimore D. 1971; Effect of pactamycin on synthesis of poliovirus proteins: a method for genetic mapping. Journal of Virology 8:395–401
    [Google Scholar]
  31. Takemoto K. K., Habel K. 1959; Sensitivity and resistance of type 1 polioviruses to an inhibitor in certain horse sera. Virology 9:228–243
    [Google Scholar]
  32. Takemoto K. K., Liebhaber H. 1962; Virus-polysaccharide interactions. II. Enhancement of plaque formation and the detection of variants of poliovirus with dextran sulphate. Virology 17:499–501
    [Google Scholar]
  33. Tannock G. A., Gibbs A. J., Cooper P. D. 1970; A re-examination of the molecular weight of poliovirus RNA. Biochemical and Biophysical Research Communications 38:298–304
    [Google Scholar]
  34. Wentworth B. B., Mccahon D., Cooper P. D. 1968; Production of infectious RNA and serum-blocking antigen by poliovirus temperature-sensitive mutants. Journal of General Virology 2:297–307
    [Google Scholar]
  35. Yamazi Y., Takahashi M., Todome Y. 1970; Inhibition of poliovirus by effect of a methylthiopyrimidine derivative. Proceedings of the Society for Experimental Biology and Medical Science 133:674–677
    [Google Scholar]
  36. Yogo Y., Wimmer E. 1972; Polyadenylic acid at the 3′-terminus of poliovirus RNA. Proceedings of the National Academy of Sciences of the United States of America 69:1877–1882
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-29-1-109
Loading
/content/journal/jgv/10.1099/0022-1317-29-1-109
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error