1887

Abstract

SUMMARY

The effect of interferon on expression of Epstein–Barr virus (EBV) early gene functions was investigated. The ‘early antigen’ synthesis which follows either EBV superinfection of established lymphoid cell lines or 5′-iododeoxyuridine activation of the intrinsic EBV genomes harboured by these cells could be suppressed with interferon. In contrast, the spontaneous early antigen expression that occurs in a few per cent of the cells in the producer cell lines could not be blocked with interferon.

The lymphoid cell lines tested differed in their ability to acquire an antiviral state after exposure to interferon. Several cell lines were also growth inhibited by the interferon preparations. The antiviral and growth inhibitory activities of different interferon preparations could not be separated by a number of criteria.

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1975-08-01
2024-10-09
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References

  1. Adams A. 1973; Concentration of Epstein–Barr virus from cell culture fluids with polyethylene glycol. Journal of General Virology 20:391–394
    [Google Scholar]
  2. Adams A., Klein G. 1973; Superinfection with Epstein–Barr virus of human lymphoid cell lines of different origins. Nature New Biology 242:234–236
    [Google Scholar]
  3. Adams A., Lindahl T. 1975; Epstein–Barr virus genomes with properties of circular DNA molecules in carrier cells. Proceedings of the National Academy of Sciences of the United States of America (in the press)
    [Google Scholar]
  4. Adams A., Lidin B., Strander H., Cantell K. 1975; Spontaneous interferon production and Epstein–Barr virus antigen expression in human lymphoid cell lines. Journal of General Virology 28:219–223
    [Google Scholar]
  5. Cantell K., Pyhala L. 1973; Circulating interferon in rabbits after administration of human interferon by different routes. Journal of General Virology 20:97–104
    [Google Scholar]
  6. Cantell K., Hirvonen S., Mogensen K. E., Pyhälä L. 1974; Human leukocyte interferon: production, purification, stability, and animal experiments. In The production and use of interferon for the treatment and prevention of human virus infections pp 35–38 In Vitro, Monograph No. 3
    [Google Scholar]
  7. Clarkson B., Striss A., De Harven E. 1967; Continuous culture of seven new lines (SKL-i to 7) from patients with acute leukemia. Cancer 20:920–947
    [Google Scholar]
  8. Durr F. E., Monroe J. H., Schmitter R., Traul K. A., Hirshaut Y. 1970; Studies on the infectivity and cytopathology of Epstein–Barr virus in human lymphoblastoid cells. International Journal of Cancer 6:436–449
    [Google Scholar]
  9. Gerber P. 1972; Activation of EBV by 5-bromodeoxyuridine in ‘virus-free’ human cells. Proceedings of the National Academy of Sciences of the United States of America 69:83–85
    [Google Scholar]
  10. Gergely L., Klein G., Ernberg I. 1971; The action of DNA antagonists on Epstein–Barr virus (EBV)-associated early antigen (EA) in Burkitt lymphoma lines. International Journal of Cancer 7:293–302
    [Google Scholar]
  11. Gresser I. 1972; Antitumor effects of interferon. Advances in Cancer Research 16:97–140
    [Google Scholar]
  12. Gresser I. 1974; Antitumor effects of interferon. Conference on ‘Modulation of host immune resistance in the prevention or treatment of induced neoplasias Bethesda; Maryland:9–11 December (in the press)
    [Google Scholar]
  13. Gresser I., Falcoff R., Fontaine-Brouty-Boye D., Zajdela F., Coppey J., Falcoff E. 1967; Interferon and murine leukemia. IV. Further studies on the efficacy of interferon preparations administered after inoculation of Friend virus. Proceedings of the Society for Experimental Biology and Medicine 126:791–797
    [Google Scholar]
  14. Hinuma Y., Grace J. T. 1967; Cloning of immunoglobulin-producing human leukemia and lymphoma cells in long term cultures. Proceedings of the Society for Experimental Biology, New York 124:107–111
    [Google Scholar]
  15. Ikawata S., Grace J. T. 1964; Cultivation in vitro of myeloblasts from human leukemia. New York State Medical Journal 64:2279–2282
    [Google Scholar]
  16. International Symposium on Standardization of Interferon and Interferon Inducers, London 1969 Symp. Ser. Immunol. Standard 14326 Basel and New York: Karger;
  17. Junge V., Hoekstra J., Deinhardt F. 1971; Stimulation of peripheral lymphocytes by allogeneic and autochthonous mononucleosis lymphocyte cell lines. Journal of Immunology 106:1306–1315
    [Google Scholar]
  18. Klein E., Klein G., Nadkarni J. S., Nadkarni J. J., Wigzell H., Clifford P. 1967; Surface IgM kappa specificity on a Burkitt lymphoma cell in vitro and in derived culture lines. Cancer Research 28:1300–1310
    [Google Scholar]
  19. Klein G. 1973; The Epstein–Barr virus (EBV). In The Herpesviruses pp 521–555 Edited by Kaplan A. New York: Academic Press;
    [Google Scholar]
  20. Klein G., Dombos L. 1973; Relationship between the sensitivity of EBV-carrying lymphoblastoid lines to superinfection and the inducibility of the resident viral genome. International Journal of Cancer 11:327–337
    [Google Scholar]
  21. Klein G., Dombos L., Gothoskar B. 1972; Sensitivity of Epstein–Barr virus (EBV) producer and nonproducer human lymphoblastoid cell lines to superinfection with EB-virus. International Journal of Cancer 10:44–57
    [Google Scholar]
  22. Lindahl T., Klein G., Reedman B. M., Johansson B., Singh S. 1974; Relationship between Epstein–Barr virus (EBV) DNA and the EBV determined nuclear antigen (EBNA) in Burkitt lymphoma biopsies and other lymphoproliferative malignancies. International Journal of Cancer 13:764–772
    [Google Scholar]
  23. Lockart R. Z. 1973; Interference and interferon with respect to herpesviruses. In The Herpesviruses pp 262–269 Edited by Kaplan A. New York: Academic Press;
    [Google Scholar]
  24. Miller G. 1971; Human lymphoblastoid cell lines and Epstein–Barr virus: a review of their interrelationships and their relevance to the etiology of leukoproliferative states in man. Yale Journal of Biology and Medicine 43:358–384
    [Google Scholar]
  25. Miller G. 1974; The oncogenicity of Epstein–Barr virus. Journal of Infectious Diseases 130:187–205
    [Google Scholar]
  26. Mogensen K. E., Cantell K. 1973; Stability of human leukocyte interferon towards heat. Acta Pathologica Microbiologica Scandinavica 81:382–383
    [Google Scholar]
  27. Mogensen K. E., Cantell K. 1974a; Human leukocyte interferon: a role for disulphide bonds. Journal of General Virology 22:95–103
    [Google Scholar]
  28. Mogensen K. E., Cantell K. 1974b; Loss of specific antigenicity on carboxymethylation of mercapto-ethanol-reduced interferon of human leukocyte origin. Intervirology 2:52–55
    [Google Scholar]
  29. Nonoyama M., Pagano J. S. 1973; Homology between Epstein–Barr virus DNA and viral DNA from Burkitt’s lymphoma and nasopharyngeal carcinoma determined by DNA-DNA reassociation kinetics. Nature, London 242:44–47
    [Google Scholar]
  30. Oxman M. N. 1973; Interferon, tumors and tumor viruses. In Interferons and Interferon Inducers pp 391–480 Edited by Finter N. B. Amsterdam: North Holland Publishing Company;
    [Google Scholar]
  31. Pulvertaft R. J. V. 1965; A study of malignant tumours in Nigeria by short term tissue culture. Journal of Clinical Pathology 18:261–273
    [Google Scholar]
  32. Reedman B. M., Klein G. 1973; Cellular localization of an Epstein–Barr virus (EBV)-associated complement fixing antigen in producer and non-producer lymphoblastoid cell lines. International Journal of Cancer 11:499–520
    [Google Scholar]
  33. Strander H., Cantell K. 1966; Production of interferon by human leukocytes in vitro. Annales Medicinae Experimentalis et Biologiae Fenniae 44:265–273
    [Google Scholar]
  34. Sugawara K., Mizuno F., Osato T. 1972; Epstein–Barr virus-associated antigens in non-producing clones of human lymphoblastoid cell lines. Nature New Biology 239:242–243
    [Google Scholar]
  35. Yata J., Klein G. 1969; Some factors affecting membrane immunofluorescence reactivity of Burkitt lymphoma tissue culture cell lines. International Journal of Cancer 4:767–775
    [Google Scholar]
  36. Zur Hausen H., Diehl V., Wolf H., Schulte-Holthausen H., Schneider U. 1972; Occurrence of Epstein–Barr virus genomes in human lymphoblastoid cell lines. Nature New Biology 237189–190
    [Google Scholar]
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