High Resolution Investigations with the Scanning and Transmission Electron Microscope of Haemadsorption Binding Sites of Mumps Virus-infected HeLa Cells
Specific changes at the surface of HeLa cells infected with mumps virus were investigated in parallel with the scanning and transmission electron microscope.
The distribution of haemadsorption binding sites and virus-induced antigens at cell surfaces was simultaneously studied by labelling virus-specific antigens with peroxidase-conjugated antibodies after haemadsorption.
New information was obtained upon the three-dimensional aspect of the red blood cells, the topographical distribution of their binding sites on the infected cells, and the specific structures at the cell surface which are involved in the process of haemadsorption.
AndersonT. F.1951; Techniques for the preservation of three-dimensional structure in preparing specimens for the electron microscope. Transactions of the New York Academy of Science 13:130–133
AvrameasS.,
TernynckT.1969; The cross-linking of proteins with glutaraldehyde and its use for the preparation of immunoadsorbents. Immunochemistry 6:53–66
CohenA. L.,
MarlowD. P.,
GarnerG. E.1968; A rapid critical point method using fluorocarbons (‘Freons’) as intermediate and transitional fluids. Journal de Microscopie 7:331–342
De PetrisS.,
RaffM. C.1972; Distribution of immunoglobulin on the surface of mouse lymphoid cells as determined by immunoferritin electron microscopy. Antibody induced, temperature-dependent redistribution and its implications for membrane structure. European Journal of Immunology 2:523–535
DrzeniekR.1970; Wechselwirkungen zwischen Myxoviren und Zelloberflachen. Zeitschrift fur medizinische Mikrobiologie und Immunologie 155 315–351 156:1–38
GrahamR. C.,
KarnovskyM. J.1966; The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney. Ultrastructural cytochemistry by a new technique. Journal of Histochemistry and Cytochemistry 14:291–302
HenleW.1969 In Diagnostic Procedures for Viral and Rickettsial Infections pp 457–482 Edited by
LennetteE. H.,
SchmidtN. J.
New York: American Public Health Association Incorporated;
HotchinJ. E.,
CohenS. M.,
RuskaH.,
RuskaC.1958; Electron microscopical aspects of hemadsorption in tissue cultures infected with influenza virus. Virology 6:689–701
MannweilerK.,
RutterG.1972; Cell alterations by mumps virus and mumps virus-specific antibodies in acute and persistent virus infection in HeLa cell cultures. In Progress of Multiple Sclerosis Research and Treatment pp 16–26 Jerusalem: Israel Journal of Medical Science;
MannweilerK.,
RutterG.1973a; Comparative SEM and TEM studies on normal and virus-infected culture cells. (Attempts to label virus-specific surface antigens.). In Proceedings SEM/III pp 513–520 Edited by
JohariO.
Chicago, Illinois: IITRI;
MannweilerK.,
RutterG.1973b; Comparative investigations of virus-specific structures and antigens in the SEM, TEM and light microscope. Beiträge elektronenmikroskopischer Direktabbildungen von Oberflachen 6:445–458
MannweilerK.,
RutterG.1974; REM-Darstellung virus-induzierter Oberflächenveränderungen an Kulturzellen nach Infektion mit Influenza-Virus. Beiträge elektronenmikroskopischer Direktabbildungen von Oberflächen 7: (in the press)
RaffM. C.,
De PetrisS.1973; Movement of lymphocyte surface antigens and receptors: the fluid nature of the lymphocyte plasma membrane and its immunological significance. Federation Proceedings 32:48–54
ShelokovA.,
VogelJ. E.,
ChiL.1958; Hemadsorption (adsorption-hemagglutination) test for viral agents in tissue culture with special reference to influenza. Proceedings of the Society of Experimental Biology and Medicine 97:802–809
WesselsN. K.,
SpoonerB. S.,
AshJ. F.,
BradleyM. O.,
LuduenaM. A.,
TaylorE. L.,
WrennJ. T.,
YamadaK. M.1971; Microfilaments in cellular and developmental processes. Science, New York 171:135–143
High Resolution Investigations with the Scanning and Transmission Electron Microscope of Haemadsorption Binding Sites of Mumps Virus-infected HeLa Cells