@article{mbs:/content/journal/jgv/10.1099/0022-1317-28-1-147, author = "Adolph, K. W.", title = "Structural Transitions of Cowpea Chlorotic Mottle Virus", journal= "Journal of General Virology", year = "1975", volume = "28", number = "1", pages = "147-154", doi = "https://doi.org/10.1099/0022-1317-28-1-147", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-28-1-147", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "SUMMARY Particles of cowpea chlorotic mottle virus (CCMV) underwent an abrupt structural transition as the pH was raised to near pH 6.75. At low ionic strength (I = 0.2) the transition was observed as a decrease from 83S to 73S in the sedimentation coefficient of the virus particle. At high ionic strength (I = 1.0) the virus disassembled to components sedimenting at about 40S. These observations suggest a structural role for the RNA in maintaining virus stability. As ionic strength at pH 7.5 was increased, a critical point was reached (I = 0.4) at which the virus disassembled to the 40S aggregates. The effect of raising the temperature of both unswollen and swollen virus was to gradually, but substantially, reduce the sedimentation coefficient.", }