1887

Abstract

SUMMARY

Viruses isolated from fish with viral haemorrhagic septicaemia (), infectious haematopoietic necrosis (), spring viraemia of carp (), swim-bladder inflammation () and pike fry disease () have been grown to high titre in fathead minnow cells. While our preparations of the IHN, SVC, SBI and PFD viruses showed typical rhabdovirus morphology with bullet-shaped particles and distinct surface projections, the VHS virus preparations had a less typical rhabdovirus morphology but were pleomorphic with a preponderance of flexuous rods. Using virus labelled with [H]-uridine, it was shown that each virus contained RNA which sedimented at 38 to 40 S and was hydrolysed by very low concentrations of ribonuclease. The viruses of and had a polypeptide composition similar to that of vesicular stomatitis virus, the prototype rhabdovirus, but the and viruses gave a pattern similar to that of rabies virus. In serum neutralization tests the and viruses were indistinguishable. VHS virus showed no serological relationship with the other four viruses but there was a low level of cross-reaction between the and - viruses.

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1975-06-01
2024-10-03
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References

  1. Amend D. F., Chambers V. C. 1970; Morphology of certain viruses of salmonid fishes. 1. In vitro studies of some viruses causing hematopoietic necrosis. Journal of the Fisheries Research Board of Canada 27:1285–1293
    [Google Scholar]
  2. Amend D. F., Yasutake W. T., Mead R. W. 1969; A haematopoietic virus disease of rainbow trout and sockeye salmon. Transactions of the American Fisheries Society 98:796–804
    [Google Scholar]
  3. Bachmann P. A., Ahne W. 1973; Isolation and characterisation of agent causing swim bladder inflammation in carp. Nature, London 244:235–237
    [Google Scholar]
  4. Bachmann P. A., Ahne W. 1974; Biological properties and identification of the agent causing swim bladder inflammation in carp. Archiv für die gesamte Virusforschung 44:261–269
    [Google Scholar]
  5. Cartwright B., Talbot P., Brown F. 1970; The proteins of biologically active sub-units of vesicular stomatitis virus. Journal of General Virology, 7:267–272
    [Google Scholar]
  6. Crick J., Brown F. 1970; Small immunising sub-units in rabies virus. In The Biology of Large RNA Viruses PP. 133–140 Edited by Barry R., Mahy B. W. J. London & New York: Academic Press;
    [Google Scholar]
  7. Darlington R. W., Trafford R., Wolf K. 1972; Fish rhabdoviruses: morphology and ultrastructure of North American salmonid isolates. Archiv für die gesamte Virusforschung 39:257–264
    [Google Scholar]
  8. Fijan N., Petrinec Z., Sulimanovic D., Zwillenberg L. O. 1971; Isolation of the viral causative agent from the acute form of infectious dropsy of carp. Veterinarski archiv (Zagreb) 41:125–138
    [Google Scholar]
  9. Gravell M., Marlsberger R. G. 1965; A permanent cell line from the fathead minnow (Pimephales prometas). Annals of the New York Academy of Sciences 126:555–565
    [Google Scholar]
  10. De kinkelin P., Le berre M. 1974a; Rhabdovirus des poissons. II. Propriétés in vitro du virus de la virémie printanière de la carpe. Annales de Microbiologie (Institut Pasteur) 125 A:113–124
    [Google Scholar]
  11. De kinkelin P., Le berre M. 1974b; Nécrose hématopoiétique infectieuse des salmonidés: production d’interferon circulant induit après l’infection expérimentale de la Truite Arc-en-Ciel Salmo gairdneri. Compte rendu de l’Academie des Sciences, Paris 279:445–448
    [Google Scholar]
  12. De kinkelin P., Le berre M., Lenoir G. 1974; Rhabdovirus des poissons. I. Propriétés in vitro du virus de la maladie rouge de l’alevin de brochet. Annales de Microbiologie (Institut Pasteur) 125 A:93–111
    [Google Scholar]
  13. De kinkelin P., dorson M. 1973; Interferon production in rainbow trout (Salmo gairdneri experimentally infected with Egtved virus. Journal of General Virology 19:125–127
    [Google Scholar]
  14. De kinkelin P., Galimard B., Bootsma R. 1973; Isolation and identification of the causative agent of ‘Red Disease’ of pike (Esox lucius L. 1766). Nature, London 241:465–467
    [Google Scholar]
  15. De kinkelin P., Scherrer R. 1970; Le virus d’Egtved. I. Développement, stabilité et structure de la souche danoise F1. Annales de la Recherche Vétérinaire 1:17–30
    [Google Scholar]
  16. Lenoir G. 1973; Structural proteins of spring viraemia virus of carp. Biochemiealand Biophysical Research Communications 51:895–899
    [Google Scholar]
  17. Mccain B. B., Fryer J. L., Pilcher K. S. 1971; Antigenic relationships in a group of three viruses of salmonid fish by cross-neutralisation. Proceedings of the Society for Experimental Biology and Medicine 137:1042–1046
    [Google Scholar]
  18. Parisot T. J., Yasutake W. T., Klontz G. W. 1965; Virus diseases of salmonidae in Western United States. I. Etiology and epizootiology. New York Academy of Sciences 126:502–519
    [Google Scholar]
  19. Sokol F., Clark H. F., Wiktor T. J., Mcfalls M. L., Bishop D. H. L., Obijeski J. F. 1974; Structural phosphoproteins associated with ten rhabdoviruses. Journal of General Virology 24:433–445
    [Google Scholar]
  20. Sokol F., Stancek D., Koprowski H. 1971; Structural proteins of rabies virus. Journal of Virology 7:241–249
    [Google Scholar]
  21. Wagner R. R., Prevec L., Brown F., Summers D. F., Sokol F., Macleod R. 1972; Classification Of rhabdovirus proteins: a proposal. Journal of Virology 110:1228–1230
    [Google Scholar]
  22. Wingfield W. H., Fryer J. L., Pilcher K. S. 1969; Properties of the sockeye salmon virus (Oregon strain). Proceedings of the Society for Experimental Biology and Medicine 130:1055–1059
    [Google Scholar]
  23. Yasutake W. T. 1970; Comparative histopathology of epizootic salmonidvirus diseases. A Symposium on Diseases of Fishes and Shellfishes,. American Fisheries Society Special Publication No. 5341–350
    [Google Scholar]
  24. Zwillenberg L. O., Jensen M. H., Zwillenberg H. L. 1965; Electron microscopy of the virus of viral haemorrhagic septicaemia of rainbow trout. Archiv für die gesamte Virusforschung 17:1–19
    [Google Scholar]
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