1887

Abstract

SUMMARY

The rate of protein synthesis in chicken embryo cells infected with Sendai virus 18 to 20 h previously was about two times greater than in mock-infected controls. At this time of infection six stable virus-induced proteins, four major structural proteins ( and ) and two non-structural proteins (28K and 61K), were identified by electrophoresis in SDS-polyacrylamide gel of total cell extracts. The structural glycopeptide F was not detected in the infected cell extracts. Pulse-chase experiments showed that and 28K proteins either did not undergo any post-translational processing or the processing occurred very rapidly. By contrast, a glycopeptide was apparently derived from one of two unstable precursors, 69K or 63K, which were revealed only after a short pulse. The synthesis of virus-specific proteins appeared to be regulated since its rate varied for individual classes of proteins.

In nucleocapsid-like particles isolated from infected cells two major structural proteins ( and ) were found. A minor component with a very large mol. wt. was revealed in these particles as well as in the virus particle.

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1975-06-01
2024-03-28
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