1887

Abstract

SUMMARY

Rapid treatment of influenza virus directly on the microscope grid with non-ionic detergent has allowed better visualization of the internal component. Many micrographs show that this ribonucleoprotein () is present as a continuous strand of 6 nm diam. arranged in the form of a double coil or helix. In spite of the minimal treatment to which the virus was subjected most helices still showed signs of degradation. The findings that we have obtained lead us to suggest that the component of influenza virus must be very sensitive to both chemical and physical manipulations, any of which could cause it to fracture from one continuous strand into several pieces, although such breakages could possibly occur at specific points along its length.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-27-3-313
1975-06-01
2022-01-21
Loading full text...

Full text loading...

/deliver/fulltext/jgv/27/3/JV0270030313.html?itemId=/content/journal/jgv/10.1099/0022-1317-27-3-313&mimeType=html&fmt=ahah

References

  1. Almeida J. D., Waterson A. P. 1970 Two morphological aspects of influenza virus. In The Biology of Large RNA Viruses pp. 27–51 Edited by Barry R. D., Mahy B. W. J. London & New York: Academic Press;
    [Google Scholar]
  2. Apostolov K., Flewett T. H. 1965; Internal structure of influenza virus. Virology 26:506–508
    [Google Scholar]
  3. Barry R. D., Davies p. 1968; The sedimentation of influenza virus and its RNA in sucrose density gradients. Journal of General Virology 2:59–69
    [Google Scholar]
  4. Compans R. W., Content J., Duesberg P. H. 1972; Structure of the ribonucleoprotein of influenza virus. Journal of Virology 10:795–800
    [Google Scholar]
  5. Duesberg P. H. 1968; The RNA’s of influenza virus. Proceedings of the National Academy of Sciences of the United States of America 59:930–937
    [Google Scholar]
  6. Duesberg p. H. 1969; District subunits of the ribonucleoprotein of influenza virus. Journal of Molecular Biology 42:485–499
    [Google Scholar]
  7. Horne R. W., Waterson A. P., Wildy P., Farnham A. E. 1960; The structure and composition of the myxoviruses. I. Electron microscope studies of the structure of myxovirus particles by negative staining techniques. Virology 11:79–98
    [Google Scholar]
  8. Kingsbury D. w., Webster R. G. 1969; Some properties of influenza virus nucleocapsids. Journal of Virology 4:219–225
    [Google Scholar]
  9. Nermut M. V. 1970; Ultrastructural studies with influenza viruses. I. Effect of some reagents on the fine structure of the virion. Acta Virologica 14:185–193
    [Google Scholar]
  10. Pons M. W., Hirst G. K. 1968; Polyacrylamide gel electrophoresis of influenza virus RNA. Virology 34:386–388
    [Google Scholar]
  11. Pons M. W., Schulze I. T., Hirst G. K. 1969; Isolation and characterization of the ribonucleoprotein of influenza virus. Virology 39:250–259
    [Google Scholar]
  12. Schulze I. T. 1973; Structure of the influenza virion. In Advances in Virus Research 18 pp. 1–55 London & New York: Academic Press;
    [Google Scholar]
  13. Skehel J. J. 1971; Estimations of the molecular weight of the influenza virus genome. Journal of General Virology 11:103–109
    [Google Scholar]
  14. Skehel J. J., Schild G. C. 1971; The polypeptide composition of influenza A viruses. Virology 44:396–408
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-27-3-313
Loading
/content/journal/jgv/10.1099/0022-1317-27-3-313
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error