RT Journal Article SR Electronic(1) A1 Still, P. E. A1 Detroy, R. W. A1 Hesseltine, C. W.YR 1975 T1 Penicillium stoloniferum Virus: Altered Replication in Ultraviolet-derived Mutants JF Journal of General Virology, VO 27 IS 3 SP 275 OP 281 DO https://doi.org/10.1099/0022-1317-27-3-275 PB Microbiology Society, SN 1465-2099, AB SUMMARY Phenotypic mutants of the wild type of Penicillium stoloniferum NRRL 5267 were obtained from conidia exposed to ultraviolet light for 60 min (10% survival). Virus content of the wild type and of nine phenotypic mutants was determined by polyacrylamide gel electrophoresis. Four mutants had no detectable Penicillium stoloniferum virus F (PsV-F), whereas the other five had levels of PsV-F in the mycelium similar to the wild-type strain. All nine mutants and the wild type had comparable levels of Penicillium stoloniferum virus S (PsV-S). Maximum virus levels occurred after 9 days of submerged culture in a 2% yeast extract-15% sucrose medium. Virus replication in the fungal host continued after protein, RNA and DNA synthesis levelled off. Virus levels ranged from 85 to 150 E 260 units (extinction units at 260 nm in 1 cm cell) per 4.7 to 5.3 g dry weight of mycelium for the mutant strains compared to 106 E 260 units per 4.2 g dry weight of the wild-type strain., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-27-3-275