1887

Abstract

SUMMARY

A number of temperature-sensitive mutants were isolated from two strains of foot-and-mouth disease virus (FMDV). Various properties of the mutants were examined including comparative growth curves at permissive and restrictive temperatures, cut-off temperatures, thermal lability and pH sensitivity. Recombination was observed between various pairs of mutants of FMDV strain Pacheco. It occurred early in the growth cycle and the proportion of recombinants remained constant thereafter. Maximum recombination was achieved if the input multiplicity of each virus was 6 p.f.u./cell or greater, provided the ratio of the input multiplicities did not vary by more than a factor of two. Day-to-day variations could be substantially reduced by normalizing recombination frequencies in terms of a standard cross. The results suggested that genetic mapping was possible with two- or three-factor crosses.

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/content/journal/jgv/10.1099/0022-1317-27-1-61
1975-04-01
2022-01-28
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References

  1. Cooper P. D. 1968; A genetic map of poliovirus temperature-sensitive mutants. Virology 35:584–596
    [Google Scholar]
  2. Lake J., MacKenzie J. S. 1973; Improved technique for the isolation of temperature-sensitive mutants of foot-and-mouth disease virus. Journal of Virology 12:665–668
    [Google Scholar]
  3. Mackenzie J. S., Dimmock N. J. 1973; A preliminary study of physiological characteristics of temperature- sensitive mutants of influenza virus. Journal of General Virology 19:51–63
    [Google Scholar]
  4. Pringle C. R. 1968; Recombination between conditional-lethal mutants within a strain of foot-and-mouth disease virus. Journal of General Virology 2:199–202
    [Google Scholar]
  5. Pringle C. R., Slade W. R., Elworthy P., O’Sullivan M. 1970; Properties of temperature-sensitive mutants of the KENYA 3/57 strain of foot-and-mouth disease virus. Journal of General Virology 6:213–220
    [Google Scholar]
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