1887

Abstract

SUMMARY

A single radial haemolysis in gel technique has been developed for the detection and measurement of antibody to influenza haemagglutinin. The method combines the sensitivity of haemagglutination-inhibition with the accuracy of single radial diffusion. It is simple, quick, reproducible, does not require purified or concentrated virus, and is unaffected by non-specific inhibitors. The method is particularly suitable for the routine screening of large numbers of serum samples, and may have application also to viruses other than the influenza group.

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/content/journal/jgv/10.1099/0022-1317-27-1-1
1975-04-01
2022-01-23
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References

  1. Expert Committee on Respiratory Virus Diseases 1959; First Report. World Health Organisation Technical Report Series. 170
    [Google Scholar]
  2. Fazekas de st. Groth S. 1949; Modification of virus receptors by metaperiodate. Australian Journal of Experimental Biology and Medicine 27:65–81
    [Google Scholar]
  3. Hiramoto R. N., Mcgee T. R., Hurst D. C., Hamlin N. M. 1971; A study of the single radial haemolysis in gel system. 1. Factors affecting the model. Immunochemistry 8:355–365
    [Google Scholar]
  4. Hirst G. K. 1942; The quantitative determination of influenza virus and antibodies by means of red cell agglutination. Journal of Experimental Medicine 75:49–64
    [Google Scholar]
  5. Kilbourne E. D. 1969; Future influenza vaccines and the use of genetic recombinants. Bulletin of the World Health Organisation 41:643–645
    [Google Scholar]
  6. Robinson R. Q., Dowdle W. R. 1969 Diagnostic Procedures for Viral and Rickettsial Diseases, 4th edition.414 New York: American Public Health Association;
    [Google Scholar]
  7. Schild G. C. 1970; Studies with antibody to the purified haemagglutinin of an influenza Ao virus. Journal of General Virology 9:191–200
    [Google Scholar]
  8. Schild G. C., Berryman I. L., Pereira H. G., Henry-Aymard M. 1972; Quantitative single-radial- diffusion tests for immunological studies with influenza virus series. Immunobiological Standardizations 20:39–46
    [Google Scholar]
  9. Schild G. C., Henry-Aymard M., Pereira H. G. 1972; A quantitative single-radial diffusion test for immunological studies with influenza virus. Journal of General Virology 16:231–236
    [Google Scholar]
  10. Weiler E., Melletz E. W., Breuningfr-Peck E. 1965; Facilitation of immune haemolysis by an interaction between red cell-sensitising antibody and globulin allotype antibody. Proceedings of the National Academy of Sciences of the United States of America 54:1310
    [Google Scholar]
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