1887

Abstract

SUMMARY

Dense poliovirus particles (buoyant density 1.44 g/ml in CsCl) isolated from infected HeLa cells contain the normal four structural polypeptides VP1 to VP4, and 35S poliovirus RNA. In addition, small amounts of VP0 and single-stranded RNA sedimenting slower than the poliovirus genome are present. Dense particles have a low specific infectivity, are neutralized by type-specific poliovirus antisera, and are detected during growth as early as normal virus but disappear when virus production stops. They appear to represent a different, more open, conformation of the normal virus capsid.

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1975-03-01
2022-01-20
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References

  1. Baltimore D. 1969 The replication of picornaviruses. In The Biochemistry of Viruses pp. 101–176 Edited by Levy H. B. New York & London: Marcel Dekker;
    [Google Scholar]
  2. Bishop I. M., Koch G. 1969a; Infectious replicative intermediate of poliovirus: purification and characterization. Virology 37:521–534
    [Google Scholar]
  3. Bishop J. M., Koch G. 1969b Plaque assay for poliovirus and poliovirus specific RNAs. In Fundamental Techniques in Virology pp. 131–145 Edited by Habel K., Salzman N. P. New York & London: Academic Press;
    [Google Scholar]
  4. Breindl M., Koch G. 1972; Competence of suspended HeLa cells for infection by inactivated poliovirus particles and by isolated viral RNA. Virology 48:136–144
    [Google Scholar]
  5. Brown F., Hull R. 1973; Comparative virology of the small RNA viruses. Journal of General Virology 20:43–60
    [Google Scholar]
  6. Cole C. N., Baltimore D. 1973a; Defective interfering particles of poliovirus. II. Nature of the defect. Journal of Molecular Biology 76:325–343
    [Google Scholar]
  7. Cole C. N., Baltimore D. 1973b; Defective interfering particles of poliovirus. III. Interference and enrichment. Journal of Molecular Biology 76:345–361
    [Google Scholar]
  8. Cole C. N., Baltimore D. 1973c; Defective interfering particles of poliovirus. IV. Mechanisms of enrichment. Journal of Virology 12:1414–1426
    [Google Scholar]
  9. Cole C. N., Smoler D., Wimmer E., Baltimore D. 1971; Defective interfering particles of poliovirus. I. Isolation And Physical Properties. Journal Of Virology 7:478–485
    [Google Scholar]
  10. Drzeniek R., Bilello P. 1972; Dissociation and reassociation of infectious poliovirus particles. Nature New Biology 240:118–122
    [Google Scholar]
  11. Drzeniek R., Bilello P. 1974; Absence of glycoproteins in poliovirus particles. Journal of General Virology 25:125–132
    [Google Scholar]
  12. Enzyme Nomenclature 1973 Recommendations (1972) of the International Union of Pure and Applied Chemistry and the International Union of Biochemistry. Amsterdam: Elsevier;
    [Google Scholar]
  13. Fenwick M. L., Wall M. J. 1972 The density of poliovirus-specific polysomes. Journal of General Virology 17143–146
    [Google Scholar]
  14. Fernandez-Tomas C. B., Baltimore D. 1973; Morphogenesis of poliovirus. II. Demonstration of a new intermediate, the provirion. Journal of Virology 12:1122–1130
    [Google Scholar]
  15. Huang A. S., Baltimore D. 1970; Initiation of polyribosome formation in poliovirus-infected HeLa cells. Journal of Molecular Biology 47:275–291
    [Google Scholar]
  16. Koch G. 1971a; Stability of polycation-induced cell competence for infection by viral RNA. Virology 45:841–843
    [Google Scholar]
  17. Koch G. 1971b; Differential effect of phleomycin on the infectivity of poliovirus and poliovirus-induced ribonucleic acids. Journal of Virology 8:28–34
    [Google Scholar]
  18. Korant B. D., Lonberg-Holm K. K., Halperen S. 1970; Structural polypeptides of three rhinoviruses. Biochemical And Biophysical Research Communications 41:477–481
    [Google Scholar]
  19. Korant B. D., Stasny J. T. 1973; Crystallization of human rhinovirus 1 A. Virology 55:410–417
    [Google Scholar]
  20. Maizel J. V. 1971 Polyacrylamide gel electrophoresis of viral proteins. In Methods in Virology v pp. 179–244 Edited by Maramorosch K., Koprowski H. New York & London: Academic Press;
    [Google Scholar]
  21. Maizel J. V., Philips B. A., Summers D. F. 1967; Composition of artificially produced and naturally occurring empty capsids of poliovirus type i. Virology 32:692–699
    [Google Scholar]
  22. Mandel B. 1962; The use of sodium dodecyl sulfate in studies on the interaction of poliovirus and HeLa-cells. Virology 17:288–294
    [Google Scholar]
  23. Miller A. O. 1972; Study of RNA extracted from HeLa cell polysomes. Isopycnic centrifugation of cytoplasmic particles extracted from normal, poliovirus-infected HeLa cells and mouse A9 cells. Archives of Biochemistry and Biophysics 150:282–295
    [Google Scholar]
  24. Philipson L. 1967 Water-organic solvent phase systems. In Methods in Virology 11 pp. 235–244 Edited by Maramorosch K., Koprowski H. New York & London: Academic Press;
    [Google Scholar]
  25. Rueckert R. R. 1971 Picornaviral architecture. In Comparative Virology pp. 255–306 Edited by Maramorosch K., Kurstak E. New York & London: Academic Press;
    [Google Scholar]
  26. Sangar D. V., Rowlands D. J., Smale C. I., Brown F. 1973; Reaction of glutaraldehyde with foot-and-mouth disease virus. Journal of General Virology 21:399–406
    [Google Scholar]
  27. Summers D. F., Maizel J. V., Darnell J. E. 1965; Evidence for virus-specific noncapsid proteins in poliovirus-infected HeLa-cells. Proceedings of the National Academy of Sciences of the United States of America 54:505–513
    [Google Scholar]
  28. Wiegers K. J., Koch G. 1972; Interaction of poliovirus-induced double-stranded RNA with HeLa-cells. Archives of Biochemistry and Biophysics 148:89–96
    [Google Scholar]
  29. Wouters M., Miller A. O. A., Fenwick M. L. 1973; Distortion of poliovirus particles by fixation with formaldehyde. Journal of General Virology 18:211–214
    [Google Scholar]
  30. Wright P. J., Cooper P. D. 1974; Poliovirus proteins associated with ribosomal structures in infected cells. Virology 59:1–20
    [Google Scholar]
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