1887

Abstract

SUMMARY

RNA was extracted from primary chicken embryo kidney (CEK) cells infected with chicken embryo lethal orphan (CELO) virus and exposed to a pulse of [5-H]-uridine late in infection. When this RNA was self-annealed, 4.5% became resistant to pancreatic ribonuclease digestion. The ribonuclease-resistant RNA was isolated by chromatography on Sephadex G-100, and the RNA was found to have the characteristics of a double-stranded molecule of sedimentation coefficient 8 S. Half of the column-isolated RNA hybridized to CELO DNA with equal amounts of virus RNA binding to the heavy or light stands of the CELO DNA, indicating the presence of complementary RNA species late in the infectious cycle of CELO.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-26-2-209
1975-02-01
2022-12-04
Loading full text...

Full text loading...

/deliver/fulltext/jgv/26/2/JV0260020209.html?itemId=/content/journal/jgv/10.1099/0022-1317-26-2-209&mimeType=html&fmt=ahah

References

  1. Aloni Y. 1972; Extensive transcription of simian virus 40 DNA in virus-yielding cells. Proceedings of the National Academy of Sciences of the United States of America 69:2404
    [Google Scholar]
  2. Aloni Y. 1973; PolyA and symmetrical transcription of SV40 DNA. Nature New Biology 234:2
    [Google Scholar]
  3. Aloni Y., Locker H. 1973; Symmetrical in vivo transcription of polyoma DNA and the separation of selfcomplementary viral and cell RNA. Virology 54:495
    [Google Scholar]
  4. Bakay M., Burke D. C. 1972; The production of interferon in chick cells infected with DNA viruses: a search for double-stranded RNA. Journal of General Virology 16:399
    [Google Scholar]
  5. Bøvre K., Szybalski W. 1969; Patterns of convergent and overlapping transcription with the coliphage λ. Virology 38:614
    [Google Scholar]
  6. Colby C., Duesberg P. H. 1969; Double-stranded RNA in vaccinia infected cells. Nature, London 222:940
    [Google Scholar]
  7. Colby C., Jurale C., Kates J. R. 1971; Mechanism of synthesis of vaccinia virus double-stranded ribonucleic acid in vivo and in vitro. Journal of Virology 7:71
    [Google Scholar]
  8. Freidrich R., Feix G. 1972; RNA-RNA hybridization in aqueous solutions containing formamide. Analytical Biochemistry 50:467
    [Google Scholar]
  9. Gillespie D., Spiegelman S. 1965; A quantitative assay for DNA-RNA hybrids with DNA immobilized in a filter. Journal of Molecular Biology 12:829
    [Google Scholar]
  10. Jurale C., Kates J. R., Colby C. 1970; Isolation of double-stranded RNA from T4 phage-infected cells. Nature, London 226:1027
    [Google Scholar]
  11. Kimball P. C., Duesberg P. H. 1971; Virus interference by cellular double-stranded ribonucleic acid. Journal of Virology 7:697
    [Google Scholar]
  12. Laver W. G., Younghusband H. B., Wrigley N. G. 1971; Purification and properties of chick embryo lethal orphan virus. Virology 45:598
    [Google Scholar]
  13. Lucas J. J., Ginsberg H. S. 1972; Identification of double-stranded virus-specific ribonucleic acid in KB cells infected with type 2 adenovirus. Biochemical and Biophysical Research Communications 49:39
    [Google Scholar]
  14. Markovits P., Coppey J. 1972; Studies on interferon induction by the avian adenovirus CELO in chicken cells. Journal of General Virology 17:157
    [Google Scholar]
  15. Robinson A. J., Bellett A. J. D. 1974 The complementary strands of CELO virus DNA. (in the press)
    [Google Scholar]
  16. Somers K. D., May J. T., Kit S. 1973; Control of gene expression in rat cells transformed by a cold-sensitive murine saecoma (MSV) mutant. Intervirology 1:176
    [Google Scholar]
  17. Younghusband H. B., Bellett A. J. D. 1971; Mature form of the deoxyribonucleic acid from chick embryo lethal orphan virus. Journal of Virology 8:265
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-26-2-209
Loading
/content/journal/jgv/10.1099/0022-1317-26-2-209
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error