Electron Microscopic Studies on Assembly of Herpes Simplex Virus upon Removal of Hydroxyurea Block Free

Abstract

SUMMARY

The release of hydroxyurea-treated, herpes simplex virus-infected cells from the drug-induced block resulted in the prompt assembly of infectious virus. Electron microscope observations at sequential intervals following removal of the drug revealed considerable synchrony of replication. This synchrony permitted stages in the complex process of core assembly to be examined in detail. The data suggest that after partial or complete assembly the nucleoprotein enters the differentiated capsid to become enfolded.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-26-2-171
1975-02-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/jgv/26/2/JV0260020171.html?itemId=/content/journal/jgv/10.1099/0022-1317-26-2-171&mimeType=html&fmt=ahah

References

  1. Breese S. S., DeBoer C. J. 1969; Effect of hydroxyurea on the development of African swine fever virus. American Journal of Pathology 55:69–77
    [Google Scholar]
  2. Dove W. F. 1966; Action of the lambda chromosome. I. Control of functions late in bacteriophage development. Journal of Molecular Biology 19:187–201
    [Google Scholar]
  3. Frankel F. R. 1968; DNA replication after T4 infection. Cold Spring Harbor Symposia on Quantitative Biology 33:485–493
    [Google Scholar]
  4. Furlong D., Swift H., Roizman B. 1972; Arrangement of herpesvirus deoxyribonucleic acid in the core. Journal of Virology 10:1071–1074
    [Google Scholar]
  5. Gibson W., Roizman B. 1972; Proteins specified by herpes simplex virus. VII. Characterization and composition of multiple capsid forms of sub types 1 and 2. Journal of Virology 10:1044–1052
    [Google Scholar]
  6. Jasty V., Chang P. w. 1970; Effect of hydroxyurea on replication of infectious bovine rhinotracheitis virus. American Journal of Veterinary Research 31:1943–1949
    [Google Scholar]
  7. King J., Lenk E. V., Botstein D. 1973; Mechanism of head assembly and DNA encapsulation in salmonella phage P22. II. Morphogenetic pathway. Journal of Molecular Biology 80:697–731
    [Google Scholar]
  8. Laemmli U. K., Favre M. 1973; Maturation of head of bacteriophage T4. I. DNA packaging events. Journal of Molecular Biology 80:575–599
    [Google Scholar]
  9. Levy J. A., Huebner R. J., Kern J., Gilden R. V. 1968; High titre T-antigen with minimal amounts of structural antigen in adenovirus-infected cells treated with hydroxyurea. Nature, London 217:744–745
    [Google Scholar]
  10. Luftig R. B., Wood W. B., Okinaka R. 1971; Bacteriophage T4 head morphogenesis. On the nature of gene 4 defective heads and their role as intermediates. Journal of Molecular Biology 57:555–573
    [Google Scholar]
  11. Mccombs R. M., Brunschwig J. P., Mirkovic R., Beyesh-Melnick. 1971; Electron microscopic characterization of herpes like virus isolated from tree shrews. Virology 45:816–820
    [Google Scholar]
  12. Miyamoto K., Morgan C. 1971; Structure and development of viruses observed in the electron microscope. XI. Entry and uncoating of herpes simplex virus. Journal of Virology 8:910–918
    [Google Scholar]
  13. Morishima I., Romano T., Onodera K. 1971; Effect of hydroxyurea on the development of bacteriophage ϕ X 174. Agricultural & Biological Chemistry 35:143–149
    [Google Scholar]
  14. Nazerian K., Lee K. F., Witter R. L., Burmester B. R. 1971; Ultrastructure studies of a herpesvirus of turkeys antigenically related to Marek’s disease virus. Virology 43:442–452
    [Google Scholar]
  15. Nii S. 1971; Electron microscopic observations on FL cells infected with herpes simplex virus. I. Viral forms. Bikens Journal 14:177–190
    [Google Scholar]
  16. Nii S., Morgan C., Rose H. M. 1968; Electron microscopy of herpes simplex virus. II. Sequence of development. Journal of Virology 2:517–536
    [Google Scholar]
  17. Nii S., Rosenkranz H. S., Morgan C., Rose H. M. 1968; Electron microscopy of herpes simplex virus. III. Effect of hydroxyurea. Journal of Virology 2:1163–1171
    [Google Scholar]
  18. Nordemskjold B. A., Krakoff I. H. 1968; Effect of hydroxyurea on polyoma virus replication. Cancer Research 28:1686–1691
    [Google Scholar]
  19. Rosenkranz H. S., Becker Y. 1973; Reversible inhibition of herpes simplex virus replication of hydroxyurea. Antimicrobial Agents and Chemotherapy 3:325–331
    [Google Scholar]
  20. Salzman L. A., Weissbach A. 1967; Formation of intermediates in the replication of phage lambda DNA. Journal of Molecular Biology 28:53–70
    [Google Scholar]
  21. SimoN L. D. 1972; Infection of Escherichia coli by T2 and T4 bacteriophages as seen in the electron microscope. Proceedings of the National Academy of Sciences of the United States of America 69:907–911
    [Google Scholar]
  22. Zweig M., Rosenkranz H. S., Morgan C. 1972; Development of coliphage T5: ultrastructural and biochemical studies. Journal of Virology 9:526–543
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-26-2-171
Loading
/content/journal/jgv/10.1099/0022-1317-26-2-171
Loading

Data & Media loading...

Most cited Most Cited RSS feed