1887

Abstract

SUMMARY

The kinetics of formation, the stability at 40 °C and the serological properties of thymidine kinase and deoxycytidine kinase activities induced by herpes simplex virus have been examined. The results are consistent with the hypothesis that both activities are carried on the same molecule − a deoxypyrimidine kinase.

Mutants deficient in deoxypyrimidine kinase have been used to produce, by absorption of general antisera, deoxypyrimidine kinase-specific antisera. Using immunoprecipitation and SDS-polyacrylamide gel electrophoresis, only one size of polypeptide (mol. wt. 42400 ± 200) has been found, constituting the type 2 enzyme.

This is close to published values for the type 1 enzyme but co-electrophoresis demonstrated that the polypeptide of the type 1 enzyme was slightly bigger.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-26-2-159
1975-02-01
2022-01-22
Loading full text...

Full text loading...

/deliver/fulltext/jgv/26/2/JV0260020159.html?itemId=/content/journal/jgv/10.1099/0022-1317-26-2-159&mimeType=html&fmt=ahah

References

  1. Beale A. J., Christofinis G. C., Furminger I. G. S. 1963; Rabbit cells susceptible to rubella virus. Lancet ii:640–641
    [Google Scholar]
  2. Buchan A., Luff S., Wallis C. 1970; Failure to demonstrate the interaction of subunits of thymidine kinase in cells simultaneously infected with herpes virus and a kinaseless mutant. Journal of General Virology 9:239–242
    [Google Scholar]
  3. Davis B. J. 1964; Disc electrophoresis. II. Method and application to human serum proteins. Annals of the New York Academy of Sciences 121:404–427
    [Google Scholar]
  4. Dimmock N. J., Watson D. H. 1969; Proteins specified by influenza virus in infected cells: analysis by polyacrylamide gel electrophoresis of antigens not present in the virus particle. Journal of General Virology 5:499–509
    [Google Scholar]
  5. Dubbs D. R., Kit S. 1964; Mutant strains of herpes simplex deficient in thymidine kinase-inducing ability Virology. 22:493–502
    [Google Scholar]
  6. Hay J., Perera P. A. J., Morrison J. M., Gentry G. A., Subak-Sharpe J. H. 1971 Herpes virus Specified proteins. In Strategy of the Viral Genome pp. 355–372 Edited by Wolstenholme G. E. W., O’Connor M. Edinburgh & London: Churchill Livingstone;
    [Google Scholar]
  7. Honess R. W., Watson D. H. 1974a; Herpes simplex virus-specific polypeptides studied by polyacrylamide gel electrophoresis of immune precipitates. Journal of General Virology 22:171–185
    [Google Scholar]
  8. Honess R. W., Watson D. H. 1974b; Absence of a requirements for host polypeptides in the herpes virus thymidine kinase. Journal of General Virology 24:215–220
    [Google Scholar]
  9. Jamieson A. T., Subak-Sharpe J. H. 1974; Biochemical studies on the herpes simplex virus specified de-oxypyrimidine kinase activity. Journal of General Virology 24:481–492
    [Google Scholar]
  10. Jamieson A. T., Gentry G. A., Subak-Sharpe J. H. 1974; Induction of both thymidine and deoxycytidine kinase activity by herpes viruses. Journal of General Virology 24:465–480
    [Google Scholar]
  11. Klemperer H. G., Haynes G. R., Shedden W. I. H., Watson D. H. 1967; A virus-specific thymidine kinase in BHK 21 cells infected with herpes simplex virus. Virology 31:120–128
    [Google Scholar]
  12. Ogino T., Shiman R., Rapp F. 1973; Deoxythymidine kinase from rabbit kidney cells infected with herpes simplex virus types 1 and 2. Intervirology 1:80–95
    [Google Scholar]
  13. Ornstein L. 1964; Disc electrophoresis. I. Background and theory. Annals of the New York Academy of Sciences 121:321–349
    [Google Scholar]
  14. Russell W. C. 1962; A sensitive and precise plaque assay for herpes virus. Nature, London 195:1028–1029
    [Google Scholar]
  15. Thouless M. E. 1972; Serological properties of thymidine kinase produced in cells infected with type 1 or type 2 herpes virus. Journal of General Virology 17:307–315
    [Google Scholar]
  16. Thouless M. E., Skinner G. R. B. 1971; Differences in the properties of thymidine kinase produced in cells infected with type 1 or type 2 herpes virus. Journal of General Virology 12:195–197
    [Google Scholar]
  17. Vantsis T. J., Wildy P. 1962; Interaction of herpes virus and HeLa cells: Comparison of cell killing and infective center formation. Virology 17:225–232
    [Google Scholar]
  18. Watson D. H., Shedden W. I. H., Elliot A., Tetsuka T., Wildy P., Bourgaux-Ramoisy D., Gold E. 1966; Virus specific antigens in mammalian cells infected with herpes simplex virus. Immunology 11:399–408
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-26-2-159
Loading
/content/journal/jgv/10.1099/0022-1317-26-2-159
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error