1887

Abstract

SUMMARY

Satellite or defective bacteriophage particles can appear in extensively recycled stocks of coliphage M13. These particles, herein known as miniphage, replicate using the wild type bacteriophage as a helper. Their physical properties (u.v. spectra, sedimentation of DNA and bacteriophage, electrophoretic mobility) are described and a method for the isolation of specific satellite bacteriophage is presented.

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/content/journal/jgv/10.1099/0022-1317-26-1-87
1975-01-01
2024-04-25
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References

  1. Benzinger R., Delius H., Jaenisch R., Hofschneider P. H. 1967; Preparation and properties of E. coli spheroplasts, competent to infection by phage nucleic acids. European Journal of Biochemistry 2:414–428
    [Google Scholar]
  2. Braunitzer G., Asbeck F., Beyreuther K., Kohler H., Von Wettstein G. 1967 Coat protein Sequence of fd. In Molecular Genetics274–284 Edited by Wittman H. G., Schuster H. New York: Springer Verlag;
    [Google Scholar]
  3. Davis R. W., Simon M., Davidson N. 1971 Electron microscope meteroduplex methods for mapping regions of base sequence homology in nucleic acids. In Nucleic Acids in Methods in Enzymology vol. 21413–428 Edited by Colowick S. P., Kaplan N. O. New York & London: Academic Press;
    [Google Scholar]
  4. Day L. A. 1969; Conformations of single stranded DNA and coat protein in fd bacteriophage as revealed by ultra-violet absorption spectroscopy. Journal of Molecular Biology 39:265–277
    [Google Scholar]
  5. Grandis A. S., Webster R. E. 1973; A new species of covalently closed fi DNA in Escherichia coli infected with an amber mutant of bacteriophage fi. Virology 55:14–19
    [Google Scholar]
  6. Griffith J. D., Kornberg A. 1974 Mini M13; circular fragments of M13 DNA replicated and packaged during normal M13 infections. Virology (in the press)
    [Google Scholar]
  7. Huang A. S., Baltimore D. 1970 Defective viral particles and viral disease processes. Nature, London 226325–327
    [Google Scholar]
  8. Knippers R., Hoffman-Berling H. 1966; A coat protein of bacteriophage fd. I−III. Specificity of coat protein DNA associations in vitro. Journal of Molecular Biology 21:281–312
    [Google Scholar]
  9. Marvin D. A., Hohn B. 1969; Filamentous bacterial viruses. Bacteriological Reviews 33:172–209
    [Google Scholar]
  10. Marvin D. A., Schaller H. 1969; The topology of the DNA from the small filamentous bacteriophage fd. Journal of Molecular Biology 15:1–7
    [Google Scholar]
  11. Pratt D., Tzagoloff H., Erdahl W. S. 1966; Conditional lethal mutants of the small filamentous coli-phage M13.1. Isolation, complementation, cell killing, time of cistron action. Virology 30:397–410
    [Google Scholar]
  12. Ray D. S., Schekman R. W. 1970; Replication of Bacteriophage M13.1. Sedimentation analysis of crude lysates of M13 infected cells. Biochimica et biophysica acta 179:398–407
    [Google Scholar]
  13. Rossomando E. F., Zinder N. D. 1968; Studies on bacteriophage fi.i. Alkali induced disassembly of the phage into DNA and protein. Journal of Molecular Biology 36:387–399
    [Google Scholar]
  14. Salivar W. O., Tzagoloff H., Pratt D. 1967; Purification and properties of diploid particles of coliphage M13. Virology 32:41–51
    [Google Scholar]
  15. Schekman R. W., Wickner W., Westergaard O., Brutlag D., Geider K., Bertsch L. L., Kornberg A. 1972; Initiation of DNA synthesis: synthesis of ϕX174 RF requires RNA synthesis resistant to Rifampicin. Proceedings of the National Academy of Sciences of the United States of America 69:2691–2695
    [Google Scholar]
  16. Yamamoto K. R., Alberts B., Benzinger R., Lawhorne L., Treiber G. 1970; Rapid bacteriophage sedimentation in the presence of polyethylene glycol and its application to large scale virus purification. Virology 40:734–744
    [Google Scholar]
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