1887

Abstract

SUMMARY

Four temperate phages (PB2A, PB6(Ω), PV-1(LV-1) and PS8) were shown to have the same genome size. Moreover hybridization experiments by the heteroduplex method and electron microscopy showed a 100% homology between these four phage genomes. Indications for lysogeny were found by direct means for the strain 396, strain 8149 and 0362 and by the electron microscope negative staining technique for the strains B6-806, B6-6, B6S3, B2AS, CV-1, 4452, 11156, 11158, 396 and 925; for strains TR-1 and 8149, the latter being bi-lysogenic, and for the 0362. These isolated phage particles, most of which appear to be defective, could be grouped into different classes. No particles could be detected in the lysates of RV3, strains 4718 and S1005, and 0363.

Further characterization by genome size was carried out for the defective temperate phages PB6-806, P4452, P8149 and P0362. No evidence for homology between PB6-806 and PB6(Ω) could be found. The defective phages PB6-806 and P4452 showed the same morphology but a different genome size, whereas the two phages P0362 and P8149 had a very different morphology and genome size.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-26-1-33
1975-01-01
2024-11-05
Loading full text...

Full text loading...

/deliver/fulltext/jgv/26/1/JV0260010033.html?itemId=/content/journal/jgv/10.1099/0022-1317-26-1-33&mimeType=html&fmt=ahah

References

  1. Adams M. H. 1959 In Bacteriophages New York: Academic Press;
    [Google Scholar]
  2. Beardsley R. E. 1955; Phage production by crown-gall bacteria and the formation of plant tumors. American Naturalist 89:175–176
    [Google Scholar]
  3. Beardsley R. E. 1960; Lysogenicity in Agrobacterium tumefaciens. Journal of Bacteriology 80:180–187
    [Google Scholar]
  4. Braun A. C. 1947; Thermal studies on the factors responsible for tumor initiation in crown-gall. American Journal of Botany 34:234–240
    [Google Scholar]
  5. Brunner M., Pootjes C. F. 1969; Bacteriophage release in a lysogenic strain of Agrobacterium tumefaciens. Journal of Virology 3:181–186
    [Google Scholar]
  6. Davis R. W., Davidson N. 1968; Electron microscopic visualization of deletion mutations. Proceedings of the National Academy of Sciences of the United States of America 60:243–250
    [Google Scholar]
  7. Davis R. W., SimoN M., Davidson N. 1971 Electron microscope heteroduplex methods for mapping regions of base sequence homology in nucleic acids. In Methods in Enzymology vol. XXI413–428 Edited by Grossman D. L., Moldave K. New York & London: Academic Press;
    [Google Scholar]
  8. De Ley J., Gillis M., Pootjes C. F., Kersters K., Tijtgat R., Van Braekel M. 1972; Relationship among temperate Agrobacterium phage genomes and coat proteins. Journal of General Virology 16:199–214
    [Google Scholar]
  9. Gillis M., De Ley J., De Cleene M. 1970; The determination of molecular weight of bacterial genome DNA from renaturation rates. European Journal of Biochemistry 12:143–153
    [Google Scholar]
  10. Kersters K., De Ley J. 1973; Numerical taxonomic analyses of Agrobacterium. Journal of General Microbiology 78:227–239
    [Google Scholar]
  11. Kleinschmidt A. K. 1968 Monolayer techniques in electron microscopy of nucleic acid molecules. In Methods in Enzymology vol. xii361–377 Edited by Grossman L., Moldave K. New York & London: Academic Press;
    [Google Scholar]
  12. Korant B. D., Pootjes C. F. 1970; Physico-chemical properties of Agrobacterium tumefaciens phage LV-1 and its DNA. Virology 40:48–54
    [Google Scholar]
  13. Kurkdjian A. 1968; Apparition de phages au cours de l’induction des tumeurs du crown-gall. Journal de Microscopie 7:1039–1044
    [Google Scholar]
  14. Kurkdjian A., Beardsley R., Manigault P. 1968; Bacteriophages d’A. tumefaciens. II. Souche B6m lysogène dans les tissus de Pisum satium L, et effet de la glycine sur la production des phages. Aannles de TInstitut Pasteur 114:554–561
    [Google Scholar]
  15. Lang D. 1970; Molecular weights of coliphages and coliphage DNA. III. Contour length and molecular weight of DNA from bacteriophages T4, T5 and T7, and from bovine papilloma virus. Journal of Molecular Biology 54:557–565
    [Google Scholar]
  16. Lippincott J. A., Beiderbeck R., Lippincott B. B. 1973; Utilization of octopine and nopaline by Agrobacterium. Journal of Bacteriology 116:378–383
    [Google Scholar]
  17. Parsons C. L., Beardsley R. E. 1968; Bacteriophage activity in homogenates of crown-gall tissue. Journal of Virology 2:651
    [Google Scholar]
  18. Pootjes C., Stemberger B. 1974; Host-phage interaction in Agrobacterium tumefaciens. II. Host strain response to mitomycin C induction. Canadian Journal of Microbiology 20:367–370
    [Google Scholar]
  19. Reynolds E. S. 1963; The use of lead citrate at high pH as an electron opaque stain in electron microscopy. Journal of Cell Biology 17:208–212
    [Google Scholar]
  20. Roussaux J., Kurkdjian A., Beardsley R. E. 1968; Bacteriophages d’Agrobacterium tumefaciens (Smith and Townsend) Conn. I. Isolement et caractères. Annales de TInstitut Pasteur 114:237–247
    [Google Scholar]
  21. SeChaud J., Kellenberger E. 1972; Electron microscopy of DNA-containing plasms. IV. Glutaralde-hyde-uranyl acetate fixation of virus-infected bacteria for thin sectioning. Journal of Ultrastructure Research 39:598–607
    [Google Scholar]
  22. Shands J. W. 1968; Embedding free-floating cells and microscopic particles; serum coagulum-epoxy resin. Stain Technology 43:15–17
    [Google Scholar]
  23. Silverman C., Schell J., Van Larebeke N., Vervliet G., Teuchy H., Van Montagu M. 1972; Studies of circular deoxyribonucleic acid molecules from bacteriophages of Agrobacterium tumefaciens. Archives Internationales de Physiologie et de Biochimie 80:409–410
    [Google Scholar]
  24. Spurr A. R. 1969; A low-viscosity epoxy resin embedding medium for electron microscopy. Journal of Ultrastructure Research 26:31–43
    [Google Scholar]
  25. Stonier T. 1956; Radioautographic evidence for the intercellular location of crown-gall bacteria. American Journal of Botany 43:647–655
    [Google Scholar]
  26. Stonier T., Mcsharry J., Speitel T. 1967; A. tumefaciens Conn. IV. Bacteriophage PB2 and its inhibitory effect on tumor induction. Virology 1:268–273
    [Google Scholar]
  27. Yamamoto K. R., Alberts B. M., Benzinger R., Lawhorne L., Treiber G. 1970; Rapid bacteriophage sedimentation in the presence of polyethylene glycol and its application to large-scale virus purification. Virology 40:734–744
    [Google Scholar]
  28. Zimmerer R. P., Hamilton R. H., Pootjes C. 1966; Isolation and morphology of temperate Agrobacterium tumefaciens bacteriophage. Journal of Bacteriology 92:746–750
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-26-1-33
Loading
/content/journal/jgv/10.1099/0022-1317-26-1-33
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error