The Multiplication of Nodamura Virus in Insect and Mammalian Cell Cultures Free

Abstract

SUMMARY

Nodamura virus multiplied in mosquito cell lines, as determined by infectivivity assays in adult honey bees () and wax moth larvae (). Titres of more than 10 and 10 bee LD/ml were obtained in culture fluids of and cells respectively after 10 days. Comparable titres were obtained after several months, during which the cultures were subdivided up to six times. Nodamura virus also multiplied in BHK cells and yielded titres of 10 to 10 mouse LD/ml and 10 to 10 wax moth LD/ml in culture fluid 1 to 4 days after infection. No c.p.e. was observed in infected cells.

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/content/journal/jgv/10.1099/0022-1317-26-1-15
1975-01-01
2024-03-29
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