%0 Journal Article %A Todd, D. %A Martin, S. J. %T Determination of the Molecular Weight of Bovine Enterovirus RNA by Nuclease Digestion %D 1975 %J Journal of General Virology, %V 26 %N 1 %P 121-129 %@ 1465-2099 %R https://doi.org/10.1099/0022-1317-26-1-121 %I Microbiology Society, %X SUMMARY The mol. wt. of a [32P]-labelled bovine enterovirus RNA has been determined by digesting with pancreatic RNase and separating the resulting oligonucleotides using a two-stage fractionation method on DEAE-Sephadex in 7 m-urea at pH 7.6 and pH 3.0. We have estimated the number of nucleotides as 8612 ± 55. This corresponds to a mol. wt. of 2.93 ± 0.02 × 106 which is in agreement with estimates obtained by sedimentation and gel electrophoresis techniques. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-26-1-121