1887

Abstract

SUMMARY

Although the infection of BHK cells with foot-and-mouth disease virus did not cause a marked inhibition of cellular protein synthesis, the proteins synthesized gradually changed from host-specific to virus-specific. The synthesis of at least thirteen virus-induced proteins was demonstrated by polyacrylamide electrophoretic analysis of the infected cells. Only a small proportion of the virus-induced proteins was incorporated into the mature virus particles. The addition of amino acid analogues caused an accumulation of the larger mol. wt. proteins, suggesting that in infected cells some cleavages occurred, giving rise to the smaller mol. wt. proteins. The operation of the precursor-cleavage mechanism was more clearly shown in pulse-chase experiments, in which it was found that the proteins were degraded from larger to smaller mol. wt.

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1975-01-01
2024-04-23
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References

  1. Baltimore D. 1969 The replication of picornaviruses. In The Biochemistry of Viruses chapter 3 101–176 Edited by Levy H. B. New York: Marcel Dekker;
    [Google Scholar]
  2. Black D. N., Brown F. 1969; Effect of actinomycin D and guanidine on the formation of a ribonucleic acid polymerase induced by foot-and-mouth disease virus and on the replication of virus and viral ribonucleic acid. Biochemical Journal 112:317–323
    [Google Scholar]
  3. Black D. N., Underwood B. O. 1970; Protein synthesis in BHK 21 cells infected with foot-and-mouth disease virus. Journal of General Virology 5:553–556
    [Google Scholar]
  4. Brown F., Martin S. J., Underwood B. O. 1966; A study of the kinetics of protein and ribonucleic acid synthesis induced by foot-and-mouth disease virus. Biochimica et Biophysica Acta 129:166–177
    [Google Scholar]
  5. Brown F., Planterose D. N., Stewart D. L. 1961; Effect of p-fluorophenylalanine on the multiplication of foot-and-mouth disease virus. Nature, London 191:414–415
    [Google Scholar]
  6. Butterworth B. E., Hall L., Stoltzfus L. M., Rueckert R. R. 1971; Virus-specific proteins synthesised in encephalomyocarditis virus infected HeLa cells. Proceedings of the National Academy of Sciences of the United States of America 68:3083–3087
    [Google Scholar]
  7. Butterworth B. E., Rueckert R. R. 1972; Kinetics of synthesis and cleavage of encephalomyocarditis virus-specific proteins. Virology 50:535–549
    [Google Scholar]
  8. Cartwright B., Talbot P., Brown F. 1970; The proteins of biologically active sub-units of vesicular stomatitis virus. Journal of General Virology 7:267–272
    [Google Scholar]
  9. Cooper P. D., Steiner-Pryor A., Wright P. J. 1973; A proposed regulator for poliovirus. The equestron. Intervirology 1:1–10
    [Google Scholar]
  10. Dubos P., Martin E. M. 1972; Virus-specific polypeptides in ascites cells infected with encephalomyocarditis virus. Journal of General Virology 17:197–212
    [Google Scholar]
  11. Dulbecco R., Vogt M. 1954; Plaque formation and isolation of pure lines with poliomyelitis viruses. Journal of Experimental Medicine 99:167–182
    [Google Scholar]
  12. Franklin R. M., Baltimore D. 1962; Patterns of macromolecular synthesis in normal and virus-infected mammalian cells. Cold Spring Harbor Symposium of Quantitative Biology 27:175–198
    [Google Scholar]
  13. Holland J. J., Kiehn E. D. 1968; Specific cleavage of viral proteins as steps in the synthesis and maturation of enteroviruses. Proceedings of the National Academy of Sciences of the United States of America 60:1015–1022
    [Google Scholar]
  14. Jacobson M. F., Asso J., Baltimore D. 1970; Further evidence on the formation of poliovirus proteins. Journal of Molecular Biology 49:657–669
    [Google Scholar]
  15. Jacobson M. F., Baltimore D. 1968; Morphogenesis of poliovirus. I. Association of the viral ribonucleic acid with coat protein. Journal of Molecular Biology 33:369–378
    [Google Scholar]
  16. Korant B. D. 1972; Cleavage of viral precursor proteins in vivo and in vitro. Journal of Virology 10:751–759
    [Google Scholar]
  17. Laporte J., Lenoir G. 1972; Inhibition des synthèses des acides ribonucleiques et des proteines cellulaires par le virus de la fièvre aphteuse. Annales de Recherches Veterinaires 3:163–174
    [Google Scholar]
  18. Macpherson I. A., Stoker M. 1962; Polyoma transformation of hamster cell clones – an investigation of genetic factors affecting cell competence. Virology 16:147–151
    [Google Scholar]
  19. Martin E. M., Kerr I. M. 1968 In The Molecular Biology of Viruses15–46 Edited by Crawford L. V., Stoker M. G. P. London: Cambridge University Press;
    [Google Scholar]
  20. McFerran J. B. 1962; Bovine enteroviruses. Annals of the New York Academy of Sciences 101:436–443
    [Google Scholar]
  21. Mowat G. N., Chapman W. G. 1962; Growth of foot-and-mouth disease virus in a fibroblastic cell line derived from hamster kidneys. Nature, London 194:253–255
    [Google Scholar]
  22. Penman D., Summers D. F. 1965; Effects on host cell metabolism following synchronous infection with poliovirus. Virology 27:614–620
    [Google Scholar]
  23. Plagemann P. G. W., Swim H. E. 1966; Replication of mengovirus. I. Effect on synthesis of macromolecules by host cell. Journal of Bacteriology 91:2317–2326
    [Google Scholar]
  24. Polatnick J., Vande Woude G. F., Arlinghaus R. B. 1968; Changes in protein and nucleic acid metabolism in baby hamster kidney cells infected with foot-and-mouth disease virus. Archiv fur die gesamte Virusforschung 23:218–226
    [Google Scholar]
  25. Summers D. F., Maizel J. V. 1968; Evidence for large precursor proteins in poliovirus synthesis. Proceedings of the National Academy of Sciences of the United States of America 59:966–974
    [Google Scholar]
  26. Summers D. F., Maizel J. V., Darnell J. E. 1965; Evidence for virus-specific non-capsid proteins in poliovirus-infected cells HeLa cells. Proceedings of the National Academy of Sciences of the United States of America 54:505–513
    [Google Scholar]
  27. Summers D. F., Shaw E. N., Stewart M. L., Maizel J. V. 1972; Inhibition of cleavage of large poliovirus-precursor proteins in infected HeLa cells by inhibitors of proteolytic enzymes. Journal of Virology 10:880–884
    [Google Scholar]
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