1887

Abstract

Summary

The major group-specific antigen (gs-a) of avian leukoviruses was detected by radioimmunoassay in extracellular medium of all embryonic cultures prepared from infected chickens. Embryos of birds not carrying an avian leukosis virus infection and of chickens from a leukosis-free flock could thus clearly be distinguished from infected ones. Release of group-specific antigen into the extracellular medium was independent of subgroup or strain of avian leukosis or Rous sarcoma virus. The radioimmunoassay of the group-specific antigen seems to be well suited to complement the COFAL (complement fixation avian leukosis) and RIF (resistance inducing factor) tests in the detection of leukosis virus in infected material.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-25-3-415
1974-12-01
2022-01-23
Loading full text...

Full text loading...

/deliver/fulltext/jgv/25/3/JV0250030415.html?itemId=/content/journal/jgv/10.1099/0022-1317-25-3-415&mimeType=html&fmt=ahah

References

  1. Allen D. W. 1969; The N-terminal amino acid of an avian leukosis group-specific antigen from avian myeloblastosis virus. Virology 38:32–41
    [Google Scholar]
  2. Allen D. W., Sarma P. S. 1972; Identification and localization of avian leukosis group-specific antigen within ‘leukosis-free’ chick embryos. Virology 48:624–626
    [Google Scholar]
  3. Armstrong D. 1969; Multiple group specific antigen components of avian tumor viruses detected with chicken and hamster sera. Journal of Virology 3:133–139
    [Google Scholar]
  4. Armstrong D., Okuyan M., Huebner R. J. 1964; Complement-fixing antigens in tissue cultures of avian leukosis viruses. Science, New York 144:1584
    [Google Scholar]
  5. Bauer H., Bolognesi D. P. 1970; Polypeptides of avian RNA tumor viruses. II. Serological characterization. Virology 42:1113–1126
    [Google Scholar]
  6. Bauer H., Schäfer W. 1966; Origin of group-specific antigen of chicken leukosis viruses. Virology 29:494–497
    [Google Scholar]
  7. Berman L. D., Sarma P. S. 1965; Demonstration of an avian leukosis group antigen by immunodiffusion. Nature, London 207:263–265
    [Google Scholar]
  8. Chen J. H., Hanafusa H. 1974; Detection of a protein of avian leukoviruses in uninfected chick cells by RIA. Journal of Virology 13:340–346
    [Google Scholar]
  9. Cook M. K., Grochal A. G., Huebner R. J. 1966; Fluorescent antibody studies of viruses of the avian leukosis complex. Journal of the National Cancer Institute 37:619–631
    [Google Scholar]
  10. Dougherty R. M., Di stefano H. S. 1966; Lack of relationship between infection with avian leukosis virus and the presence of COFAL antigen in chick embryos. Virology 29:586–595
    [Google Scholar]
  11. Dougherty R. M., Marucci A. A., Di Stefano H. S. 1972; Application of immunohistochemistry to study of avian leukosis virus. Journal of General Virology 15:149–162
    [Google Scholar]
  12. Estola T., Sandelin K., Vaheri A., Ruoslahti E., Suni J. 1974; Radioimmunoassay for detecting group-specific avian RNA tumor virus antigens and antibodies. Developments in Biological Standardization 25:115–118
    [Google Scholar]
  13. Fleissner E. 1971; Chromatographic separation and antigenic analysis of proteins of the oncorna viruses. I. Avian leukemia-sarcoma viruses. Journal of Virology 8:778–785
    [Google Scholar]
  14. Hung P. P., Robinson H. L., Robinson W. S. 1971; Isolation and characterization of proteins from Rous sarcoma virus. Virology 43:251–256
    [Google Scholar]
  15. Kawai S., Hanafusa H. 1971; The effects of reciprocal changes in temperature on the transformed state of cells infected with a Rous sarcoma virus mutant. Virology 46:470–479
    [Google Scholar]
  16. Kelloff G., Vogt P. K. 1966; Localization of avian tumor virus group-specific antigen in cell and virus. Virology 29:377–384
    [Google Scholar]
  17. Payne F. E., Solomon J. J., Purchase H. G. 1966; Immunofluorescent studies of group-specific antigen of the avian sarcoma-leukosis viruses. Proceedings of the National Academy of Sciences of the United States of America 55:341–349
    [Google Scholar]
  18. Rubin H. 1960; A virus in chick embryos which induces resistance in vitro to infection with Rous sarcoma virus. Proceedings of the National Academy of Sciences of the United States of America 46:1105–1119
    [Google Scholar]
  19. Ruoslahti E., Vaheri A., Estola T., Sandelin K. 1973; Antibodies against avian gs antigen in chickens infected naturally and experimentally with avian RNA tumor viruses. International Journal of Cancer 11:595–603
    [Google Scholar]
  20. Sarma P. S., Turner H. C., Huebner R. J. 1964; An avian leukosis group-specific complement fixation reaction. Application for the detection and assay of non-cytopathogenic leukosis viruses. Virology 23:313–321
    [Google Scholar]
  21. Suni J., Vaheri A., Ruoslahti E. 1973; Radioimmunoassay of RNA tumor virus group specific antigen. Intervirology 1:119–126
    [Google Scholar]
  22. Vaheri A., Ruoslahti E. 1973; Expression of the major group-specific antigen (gs-a) of avian type-C viruses in normal chicken cells and tissues. International Journal of Cancer 12:361–367
    [Google Scholar]
  23. Vogt P. K., Sarma P. S., Huebner J. R. 1965; Presence of avian tumor virus group-specific antigen in non-producing Rous sarcoma cells of the chicken. Virology 27:233–236
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-25-3-415
Loading
/content/journal/jgv/10.1099/0022-1317-25-3-415
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error