@article{mbs:/content/journal/jgv/10.1099/0022-1317-25-3-371, author = "Atkins, G. J. and Samuels, Janis and Kennedy, S. I. T.", title = "Isolation and Preliminary Characterization of Temperature-sensitive Mutants of Sindbis Virus Strain AR339", journal= "Journal of General Virology", year = "1974", volume = "25", number = "3", pages = "371-380", doi = "https://doi.org/10.1099/0022-1317-25-3-371", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-25-3-371", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "Summary One hundred and four temperature-sensitive mutants of Sindbis virus strain AR339 have been isolated using five different mutagens. Forty-seven mutants showed no detectable RNA synthesis at the restrictive temperature (39 °C), whereas 57 showed levels ranging from 1% to over 100% of the wild-type level. The mutants did not show complementation. The ratio of the 42S:26S RNA species in cells infected with 30 mutants showing > 10% of the wild-type level of RNA synthesis at 39 °C was measured. Of these 30 mutants, none were found which were wholly defective in production of either RNA species, although the ratio was significantly different from the wild-type for some mutants. Two mutants showed production at 39 °C of polydisperse RNA of both double-stranded and single-stranded types.", }