Newcastle disease virus (NDV) stocks contain three types of particles which were tested for interferon inducing ability: (1) plaque-forming haemagglutinating particles, (2) non-plaque-forming haemagglutinating particles containing RNA and (3) non-plaque-forming haemagglutinating particles which contain no RNA. Single NDV-infected cells were isolated in microdrops and tested for interferon production as measured by protection of 30 ± 6 additional cells from challenge with mengovirus. This technique demonstrated that: (1) essentially every cell can be induced by plaque-forming NDV to produce interferon, (2) neither of the two types of non-plaque-forming haemagglutinating particles were capable of inducing detectable levels of interferon and (3) while infection with a single active virus was sufficient to induce interferon, the efficiency of induction increased with increasing multiplicity. It was concluded that some virus synthetic processes are probably required to initiate induction.


Article metrics loading...

Loading full text...

Full text loading...


Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error