Potato virus X reacted with reagents commonly used for protein amino groups, and some of its properties were changed. 2,4,6-trinitrobenzenesulphonic acid, pyridoxal-5-phosphate and methyl picolinimidate altered its absorption spectrum; the last two altered its fluorescence spectrum, and the first two altered its electrophoretic mobility. These reagents did not necessarily inactivate the virus; preparations judged to contain two modified amino groups per protein subunit retained 50 to 100% of their initial infectivity. This supports the previous conclusion that PVX-Q, an infective product of PVX and an oxidized leaf phenol, contains modified lysine ε-amino groups.
AzegamiM.,
IwaiK.1964; Specific modification of nucleic acids and their constituents with trinitrophenyl group. The Journal of Biochemistry, Tokyo 55:346–348
BenisekW. F.,
RichardsF. M.1968; Attachment of metal-chelating functional groups to hen egg white lysozyme. The Journal of Biological Chemistry 243:4267–4271
CortijoM.,
ShaltielS.1972; On the microenvironment of the pyridoxamine 5-phosphate residue in NaBH4-reduced glycogen phosphorylase b. European Journal of Biochemistry 29:134–142
EnglanderS. W.,
EpsteinH. T.1957; Optical methods for measuring nucleoprotein and nucleic acid concentrations. Archives of Biochemistry and Biophysics 68:144–149
FinlayT. H.,
DharmgrongartamaE. D.,
PerlmannG. E.1973; Mechanism of the gossypol inactivation of pepsinogen. The Journal of Biological Chemistry 248:4827–4833
HunterM. J.,
LudwigM. L.1962; The reaction of imidoesters with proteins and related small molecules. Journal of the American Chemical Society 84:3491–3504
KoenigR.,
StegemannH.,
FrancksenH.,
PaulH. L.1970; Protein subunits in the potato virus X group. Determination of the molecular weights by polyacrylamide electrophoresis. Biochimica et Biophysica Acta 207:184–189
McKinley-McKeeJ. S.,
MorrisD. L.1972; The lysines in liver alcohol dehydrogenase. Chemical modification with pyridoxal-5-phosphate and methyl picolinimidate. European Journal of Biochemistry 28:1–11
PerhamR. N.,
RichardsF. M.1968; Reactivity and structural role of protein amino groups in tobacco mosaic virus. Journal of Molecular Biology 33:795–807
ShepardJ. F.,
SecorG. A.1972; The effects of enzymatic digestion on the molecular weight and antigenic specificity of potato virus X protein. Phytopathology 62:1154–1160
TungJ.-S.,
KnightC. A.1972; The coat protein subunits of potato virus X and white clover mosaic virus, a comparison of methods for determining their molecular weights and some in situ degradation products of potato virus X protein. Virology 49:211–223