@article{mbs:/content/journal/jgv/10.1099/0022-1317-25-2-303, author = "Pierpoint, W. S.", title = "Chemical Modification of the Lysine-Amino Groups of Potato Virus X", journal= "Journal of General Virology", year = "1974", volume = "25", number = "2", pages = "303-312", doi = "https://doi.org/10.1099/0022-1317-25-2-303", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-25-2-303", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "SUMMARY Potato virus X reacted with reagents commonly used for protein amino groups, and some of its properties were changed. 2,4,6-trinitrobenzenesulphonic acid, pyridoxal-5-phosphate and methyl picolinimidate altered its absorption spectrum; the last two altered its fluorescence spectrum, and the first two altered its electrophoretic mobility. These reagents did not necessarily inactivate the virus; preparations judged to contain two modified amino groups per protein subunit retained 50 to 100% of their initial infectivity. This supports the previous conclusion that PVX-Q, an infective product of PVX and an oxidized leaf phenol, contains modified lysine ε-amino groups.", }