1887

Abstract

SUMMARY

Using indirect immunofluorescence and hybridization techniques the synthesis of structural proteins (hexon and fibre), T-antigen and virus DNA were studied in infected rat embryo cells early after infection by adenovirus type 2. The data obtained from this study, including infectious virus yields, indicate that this is a semi-permissive system in which transformation occurs. The adenovirus type 2/rat embryo transformation system was found to be reproducible and the transformation frequency was influenced by the tissue of origin and not by the strain of rat. A comparison of the characteristics of cells transformed by either adenovirus type 2 (Ad-2) or type 12 (Ad-12) is reported. No obvious property was observed which would explain the different behaviour of rat cells transformed by these adenovirus serotypes.

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1974-11-01
2024-11-02
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References

  1. Butel J. S., Teventhia S. S., Melnick J. L. 1972; Oncogenicity and cell transformation by papovavirus SV40: the role of the viral genome. Advances in Cancer Research 15:13
    [Google Scholar]
  2. Dunn A. R., Gallimore P. H., Jones K. W., McDougall J. K. 1973; In situ hybridization of adenovirus RNA and DNA. II. Detection of adenovirus-specific DNA in transformed and tumour cells. International Journal of Cancer 11:628–636
    [Google Scholar]
  3. Freeman A. E., Black P. H., Vanderpool E. A., Henry P. H., Austin J. B., Huebner R. J. 1967; Transformation of primary rat embryo cells by adenovirus type 2. Proceedings of the National Academy of Sciences of the United States of America 58:1205–1212
    [Google Scholar]
  4. Gallimore P. H. 1972; Tumour production in immunosuppressed rats with cells transformed in vitro by adenovirus type 2. Journal of General Virology 16:99–102
    [Google Scholar]
  5. Gallimore P. H. 1973; Studies with adenovirus type 2: cellular transformation and oncogenicity. Ph.D. thesis University of Birmingham; U.K:
    [Google Scholar]
  6. Gallimore P. H., Sharp P. A., Sambrook J. 1974; Viral DNA in transformed cells. II. A study of the sequences of adenovirus 2 DNA in nine lines of transformed rat cells using specific fragments of viral genome. Journal of Molecular Biology (in the press)
    [Google Scholar]
  7. Graham F. L., van Der EB A. J. 1973; Transformation of rat cells by DNA of human adenovirus 5. Virology 54:536–539
    [Google Scholar]
  8. Hayashi K., Russell W. C. 1968; A study of the development of adenovirus antigens by the immuno-fluorescent technique. Virology 34:470–480
    [Google Scholar]
  9. Huebner R. J. 1967; Adenovirus-directed tumour and T-antigens. In Perspectives in Virology vol 5:147–166 Edited by Pollard M.
    [Google Scholar]
  10. Inbar M., Sachs L. 1969; Interaction of the carbohydrate-binding protein concanavalin A with normal and transformed cells. Proceedings of the National Academy of Sciences of the United States of America 63:1418–1425
    [Google Scholar]
  11. Mcallister R. M., Nicholson M. C., Lewis A. M., Macpherson I., Huebner R. J. 1969; Transformation of rat embryo cells by adenovirus type I. Journal of General Virology 4:29–36
    [Google Scholar]
  12. McDougall J. K., Dunn A. R., Jones K. W. 1972; In situ hybridization of adenovirus RNA and DNA. Nature, London 236:346–348
    [Google Scholar]
  13. Macpherson I., Montagnier L. 1964; Agar suspension culture for the selective assay of cells transformed by polyoma virus. Virology 23:291–294
    [Google Scholar]
  14. Orth G., Jeanteur P., Croissant O. 1970; Evidence for a localization of vegative viral DNA replication by autoradiographic detection of RNA-DNA hybrids in sections of tumours induced by Shope papilloma virus. Proceedings of the National Academy of Sciences of the United States of America 68:1876–1880
    [Google Scholar]
  15. Russell W. C., Valentine B. C., Pereira H. G. 1967; The effect of heat on the anatomy of the adenovirus. Journal of General Virology 1:509–522
    [Google Scholar]
  16. Sharp P. A., Pettersson U., Sambrook J. 1974; Viral DNA in transformed cell lines, I. A study of the sequences of adenovirus 2 DNA in a line of transformed rat cells using specific fragments of the viral genome. Journal of Molecular Biology (in the press)
    [Google Scholar]
  17. Van Der Noordaa J. 1968; Transformation of rat cells by adenovirus types 1, 2 and 3. Journal of General Virology 3:303–304
    [Google Scholar]
  18. Williams J. F. 1973; Oncogenic transformation of hamster embryo cells in vitro by adenovirus type 5. Nature, London 243:162–163
    [Google Scholar]
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