1887

Abstract

SUMMARY

The susceptibility of tobacco protoplasts to infection with cowpea chlorotic mottle virus varied with the age both of the plants and leaves used as a source for the protoplasts. The efficiency of infection for freshly prepared and stored protoplasts could be increased by an improved direct inoculation procedure. Poly--ornithine, poly--arginine, poly--lysine and poly--lysine were all effective in inducing infection, the first stage of which may occur within 20 s of exposure to inoculum. The effects of temperature, injury and sodium azide on infection suggested that the limiting process during inoculation is not controlled by enzymes. The yield of virus per infected protoplast was decreased by some antibiotics but not affected by various other additives to the culture medium.

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1974-11-01
2022-08-10
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References

  1. Aoki S., Takebe I. 1969; Infection of tobacco mesophyll protoplasts by tobacco mosaic virus ribonucleic acid. Virology 39:439–448
    [Google Scholar]
  2. Burgess J., Motoyoshi F., Fleming E. N. 1973a; Effect of poly-L-ornithine on isolated tobacco mesophyll protoplasts: evidence against stimulated pinocytosis. Planta, Berlin in:199–208
    [Google Scholar]
  3. Burgess J., Motoyoshi F., Fleming E. N. 1973b; The mechanism of infection of plant protoplasts by viruses. Planta, Berlin 112:323–332
    [Google Scholar]
  4. Cocking E. C. 1970; Virus uptake, cell wall regeneration and virus multiplication in isolated plant protoplasts. International Review of Cytology 28:89–124
    [Google Scholar]
  5. Coutts R. H. A., Cocking E. C., Kassanis B. 1972; Infection of tobacco mesophyll protoplasts with tobacco mosaic virus. Journal of General Virology 17:289–294
    [Google Scholar]
  6. Evans P. K., Woodcock J., Keates A. G. 1973; Steps towards cell fusion: studies on cereal protoplasts and protoplasts of haploid tobacco. Colloques Internationaux C.N.R.S No 212: Protoplastes et fusion de cellules somatiques vegelales 469–477 (editions de I.N.R.A. Paris)
    [Google Scholar]
  7. Hibi T., Yora K. 1972; Electron microscopy of tobacco mosaic virus infection in tobacco mesophyll protoplasts. Annals of the Phytopathological Society of Japan 38:350–356
    [Google Scholar]
  8. Motoyoshi F., Bancroft J. B., Watts J. W., Burgess J. 1973a; The infection of tobacco protoplasts with cowpea chlorotic mottle virus and its RNA. Journal of General Virology 20:177–193
    [Google Scholar]
  9. Motoyoshi F., Bancroft J. B., Watts J. W. 1973b; A direct estimate of the number of cowpea chlorotic mottle virus particles absorbed by tobacco protoplasts that become infected. Journal of General Virology 21:159–161
    [Google Scholar]
  10. Motoyoshi F., Bancroft J. B., Watts J. W. 1974; The infection of tobacco protoplasts with a variant of brome mosaic virus. Journal of General Virology 25:31–36
    [Google Scholar]
  11. Motoyoshi F., Hull R. 1974; The infection of tobacco protoplasts with pea enation mosaic virus. Journal of General Virology 24:89–99
    [Google Scholar]
  12. Otsuki Y., Takebe I. 1973; Infection of tobacco mesophyll protoplasts by cucumber mosaic virus. Virology 53:433–438
    [Google Scholar]
  13. Otsuki Y., Takebe I., Honda S., Kajita Y., Matsui C. 1974; Infection of tobacco mesophyll protoplasts by potato virus X. Journal of General Virology 22:375–385
    [Google Scholar]
  14. Otsuki Y., Takebe I., Honda Y., Matsui C. 1972; Ultrastructure of infection of tobacco mesophyll protoplasts by tobacco mosaic virus. Virology 49:188–194
    [Google Scholar]
  15. Ryser H. I-P. 1967; A membrane effect of basic polymers dependent on molecular size. Nature, London 215:934–936
    [Google Scholar]
  16. Shalla T. A., Petersen L. J. 1973; Infection of isolated plant protoplasts with potato virus X. Phytopathology 63:1125–1130
    [Google Scholar]
  17. Takebe I., Otsuki Y., Aoki S. 1968; Isolation of tobacco mesophyll cells in intact and active state. Plant and Cell Physiology 9:115–124
    [Google Scholar]
  18. Takebe L., Otsuki Y. 1969; Infection of tobacco mesophyll protoplasts by tobacco mosaic virus. Proceedings of the National Academy of Sciences of the United States of America 64:843–848
    [Google Scholar]
  19. Watts J. W., King J. M. 1973a; The metabolism of proteins and nucleic acids in freshly isolated protoplasts. Colloques Internationaux C. N. R. S No 212: Protoplastes et fusion de cellules somatiques vegetales 119–123 (editions de I. N. R. A. Paris)
    [Google Scholar]
  20. Watts J. W., King J. M. 1973b; The use of antibiotics in the culture of non-sterile plant protoplasts. Planta, Berlin 113:271–277
    [Google Scholar]
  21. Watts J. W., Motoyoshi F., King J. M. 1974; Problems associated with the production of stable proto plasts of cells of tobacco mesophyll. Annals of Botany (in the press)
    [Google Scholar]
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