1887

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Volume 25, Issue 2

Revertants of Mouse Cells Transformed by Murine Sarcoma Virus: flat Variants without a Rescuable Sarcoma Virus from a Clone of BALB/3T3 Transformed by Kirsten MSV Free

Abstract

SUMMARY

Clonal subline of Kirsten murine sarcoma virus (Ki-MSV)-transformed non-producer BALB/3T3 (Ki-BALB) cells spontaneously produced flat variants with some properties of non-transformed cells at frequencies of 0.01 to 0.001. Such variants were epithelioid, contact-inhibited, grew to low saturation density and exhibited variable cloning efficiences in soft agar. They demonstrated no murine leukaemia group-specific antigen(s), no reverse transcriptase activity, and no infectious virus, but were agglutinable by concanavalin A. Murine leukaemia virus (MuLV) was induced after 5-iododeoxyuridine (IUDR) treatment from both the flat variants and from the parental Ki-BALB cells. However, Ki-MSV could not be rescued from these flat variants by superinfection with MuLV, nor induced by treatment with IUDR. The state of reversion was stable, revealing no back-transformation during 30 to 40 subcultures. The tumourigenicity of the flat variants in BALB/c mice was markedly reduced (actually undetectable) compared to that of the Ki-BALB cells. All flat variants were susceptible to MSV and MuLV infection. The loss of the transformed phenotype in spontaneous flat variants was associated with a decrease in chromosome number to a level similar to BALB/3T3 cells. These observations suggest that these revertants may have lost some or all of the Ki-MSV genome.

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© Journal of General Virology 1974
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1974-11-01
2022-08-11
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Revertants of Mouse Cells Transformed by Murine Sarcoma Virus: flat Variants without a Rescuable Sarcoma Virus from a Clone of BALB/3T3 Transformed by Kirsten MSV
J. Gen. Virol. 25, 207 (1974); https://doi.org/10.1099/0022-1317-25-2-207
/content/journal/jgv/10.1099/0022-1317-25-2-207
/content/journal/jgv/10.1099/0022-1317-25-2-207
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