1887

Abstract

SUMMARY

Clonal subline of Kirsten murine sarcoma virus (Ki-MSV)-transformed non-producer BALB/3T3 (Ki-BALB) cells spontaneously produced flat variants with some properties of non-transformed cells at frequencies of 0.01 to 0.001. Such variants were epithelioid, contact-inhibited, grew to low saturation density and exhibited variable cloning efficiences in soft agar. They demonstrated no murine leukaemia group-specific antigen(s), no reverse transcriptase activity, and no infectious virus, but were agglutinable by concanavalin A. Murine leukaemia virus (MuLV) was induced after 5-iododeoxyuridine (IUDR) treatment from both the flat variants and from the parental Ki-BALB cells. However, Ki-MSV could not be rescued from these flat variants by superinfection with MuLV, nor induced by treatment with IUDR. The state of reversion was stable, revealing no back-transformation during 30 to 40 subcultures. The tumourigenicity of the flat variants in BALB/c mice was markedly reduced (actually undetectable) compared to that of the Ki-BALB cells. All flat variants were susceptible to MSV and MuLV infection. The loss of the transformed phenotype in spontaneous flat variants was associated with a decrease in chromosome number to a level similar to BALB/3T3 cells. These observations suggest that these revertants may have lost some or all of the Ki-MSV genome.

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1974-11-01
2022-08-11
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References

  1. Aaronson S. A. 1971; Chemical induction of focus-forming virus from nonproducer cells transformed murine sarcoma virus. Proceedings of the National Academy of Sciences of the United States of America 68:3069–3072
    [Google Scholar]
  2. Aaronson S. A., Dunn C. Y. 1974; High-frequency C-type virus induction by inhibitors of protein synthesis. Science, New York 183:422–424
    [Google Scholar]
  3. Aaronson S. A., Stephenson J. R. 1973; Independent segregation of loci for activation of biologically distinguishable RNA C-type viruses in mouse cells. Proceedings of the National Academy of Sciences of the United States of America 70:2055–2058
    [Google Scholar]
  4. Aaronson S. A., Todaro G. J. 1968; Development of 3T3-like lines from BALB/c mouse embryo cultures: transformation susceptibility to SV40. Journal of the Cell Physiology 72:141–142
    [Google Scholar]
  5. Aaronson S. A., Todaro G. J., Scolnick E. M. 1971; Induction of murine C-type viruses from clonal lines of virus-free BALB/3T3 cells. Science, New York 174:157–159
    [Google Scholar]
  6. Aaronson S. A., Weaver C. A. 1971; Characterization of murine sarcoma virus (Kirsten) transformation of mouse and human cells. Journal of General Virology 13:245–252
    [Google Scholar]
  7. Aoki T., Todaro G. J. 1973; Antigenic properties of endogenous type-C viruses from spontaneously transformed clones of BALB/3T3. Proceedings of the National Academy of Sciences of the United States of America 70:1598–1602
    [Google Scholar]
  8. Bassin R. H., Tuttle N., Fischinger P. J. 1970; Isolation of murine sarcoma virus transformed mouse cells which are negative for leukemia virus from agar suspension cultures. International Journal of Cancer 6:95–107
    [Google Scholar]
  9. Bassin R. H., Tuttle N., Fischinger P. J. 1971; Rapid cell culture assay technique for murine leukemia viruses. Nature, London 229:564–566
    [Google Scholar]
  10. Duc-Nguyen H., Rosenblum E. N., Zeigel R. F. 1966; Persistent infection of a rat kidney cell line with Rauscher leukemia virus. Journal of Bacteriology 92:1133–1140
    [Google Scholar]
  11. Fischinger P. J., Moore C. O., O’connor T. E. 1969; Isolation and identification of a helper virus found in the Moloney sarcoma-leukemia virus complex. Journal of the National Cancer Institute 42:605–622
    [Google Scholar]
  12. Fischinger P. J., Nomura S., Peebles P. T., Haapala D. K., Bassin R. H. 1972; Reversion of murine sarcoma transformed mouse cells: variants without a rescuable sarcoma virus. Science, New York 176:1033–1035
    [Google Scholar]
  13. Fischinger P. J., Nomura S., Tuttle-Fuller N., Dunn J. K. 1974; Revertants of mouse cells transformed by murine sarcoma virus. III. Metastable expression of virus functions in revertants retransformed by murine sarcoma virus. Virology 59:217–229
    [Google Scholar]
  14. Gazdar A. F., Stull H. B., Chopra H. C., Ikawa Y. 1974; Properties of flat variants of murine sarcoma virus transformed nonproducer cells isolated after high-temperature passage. International Journal of Cancer 13:219–226
    [Google Scholar]
  15. Greenberger J. S., Aaronson S. A. 1974; Morphologic revertants of murine sarcoma virus transformed nonproducer BALB/3T3: Selective techniques for isolation and biologic properties in yitro and in vivo. Virology 57:339–346
    [Google Scholar]
  16. Hartley J. W., Rowe W. P. 1966; Production of altered cell foci in tissue culture by defective Moloney sarcoma virus particles. Proceedings of the National Academy of Sciences of the United States of America 55:780–786
    [Google Scholar]
  17. Hartley J. W., Rowe W. P., Huebner R. J. 1970; Host-range restrictions of murine leukemia viruses in mouse embryo cell cultures. Journal of Virology 5:221–225
    [Google Scholar]
  18. Hatanaka M., Klein R., Toni R., Walker J., Gilden R. 1973; Mutants of nonproducer cell lines transformed by murine sarcoma viruses. I. Induction, isolation, particle production, and tumorigenicity. Journal of Experimental Medicine 138:356–363
    [Google Scholar]
  19. Hatanaka M., Klein R., Long C. W., Gilden R. 1973b; Mutants of nonproducer cell lines transformed by murine sarcoma virus. II. Relationship of tumorigenicity to presence– of viral markers and rescuable sarcoma genome. Journal of Experimental Medicine 138:364–372
    [Google Scholar]
  20. Hitotsumachi S., Rabinowitz Z., Sachs L. 1971; Chromosome control of reversion in transformed cells. Nature, London 231:511–514
    [Google Scholar]
  21. Inbar M., Rabinowitz Z., Sachs L. 1969; The formation of variants with a reversion of properties of transformed cells. III. Reversion of the structure of the cell surface membrane. International Journal of Cancer 4:690–696
    [Google Scholar]
  22. Lowy D. R., Rowe W. P., Teich N., Hartley J. W. 1971; Murine leukemia virus: High-frequency activation in vitro by 5-iododeoxyuridine and 5-bromodeoxyuridine. Science, New York 174:155–156
    [Google Scholar]
  23. Macpherson I. 1965; Reversion in hamster cells transformed by Rous sarcoma virus. Science, New York 148:1731–1733
    [Google Scholar]
  24. Macpherson I., Montagnier L. 1964; Agar suspension cultures for the selective assay of cells transformed by polyoma virus. Virology 23:291–294
    [Google Scholar]
  25. Marin G., Macpherson I. 1969; Reversion in polyoma-transformed cells: Retransformation, induced antigens and tumorigenicity. Journal of Virology 3:146–149
    [Google Scholar]
  26. Nomura S., Dunn K. J., Fischinger P. J. 1973a; Rapid screening assay for revertants derived from MSV-transformed cells. Nature, London 246:213–215
    [Google Scholar]
  27. Nomura S., Fischinger P. J., Mattern C. F. T., Gerwin B. I., Dunn K. J. 1973b; Revertants of mouse cells transformed by murine sarcoma virus. II. Flat variants induced by fluorodeoxyuridine and colcemid. Virology 56:152–163
    [Google Scholar]
  28. Nomura S., Fischinger P. J., Mattern C. F. T., Peebles P. T., Bassin R. H., Friedman G. P. 1972; Revertants of mouse cells transformed by murine sarcoma virus. I. Characterization of flat and transformed sublines without a rescuable murine sarcoma virus. Virology 50:51–64
    [Google Scholar]
  29. O’Connor T. E. 1968; Quantitative in vitro studies on a murine sarcoma-leukemia virus complex. In Perspectives in Leukemia64–80 Edited by Dameshek W., Dutcher R. M. New York: Grune & Stratton;
    [Google Scholar]
  30. Peebles P. T., Fischinger P. J., Bassin R. H., Papageorge A. G. 1973; Isolation of human amnion cells transformed by rescuable murine sarcoma virus. Nature New Biology 242:98–101
    [Google Scholar]
  31. Pollack R. E., Teebor G. W. 1969; Relationship of contact inhibition to tumor transplantability, morphology, and growth rate. Cancer Research 29:1770–1772
    [Google Scholar]
  32. Pollack R. E., Green H., Todaro G. J. 1968; Growth control in cultured cells: Selection of sublines with increased sensitivity to contact inhibition and decreased tumor-producing ability. Proceedings of the National Academy of Sciences of the United States of America 60:126–133
    [Google Scholar]
  33. Rabinowitz Z., Sachs L. 1968; Reversion of properties in cells transformed by polyoma virus. Nature, London 220:1203–1206
    [Google Scholar]
  34. Rabinowitz Z., Sachs L. 1969; The formation of variants with a reversion of properties of transformed cells. I. Variants from polyoma-transformed cells grown in vivo. Virology 38:336–342
    [Google Scholar]
  35. Rabinowitz Z., Sachs L. 1970; Control of the reversion of properties in transformed cells. Nature, London 225:136–139
    [Google Scholar]
  36. Rowe W. P., Pugh W. E., Hartley J. W. 1970; Plaque assay techniques for murine leukemia viruses. Virology 42:1136–1139
    [Google Scholar]
  37. Stephenson J. R., Aaronson S. A. 1972; A genetic locus for inducibility of C-type virus in BALB/c cells: The effect of a nonlinked regulatory gene on detection of virus after chemical activation. Proceedings of the National Academy of Sciences of the United States of America 69:2798–2801
    [Google Scholar]
  38. Stephenson J. R., Scolnick E. M., Aaronson S. A. 1972; Genetic stability of the sarcoma viruses in murine and avian sarcoma virus-transformed nonproducer cells. International Journal of Cancer 9:577–583
    [Google Scholar]
  39. Stephenson J. R., Reynolds R. K., Aaronson S. A. 1973; Characterization of morphologic revertants of murine and avian sarcoma virus-transformed cells. Journal of Virology 11:218–222
    [Google Scholar]
  40. Svoboda J. 1960; Presence of chicken tumor virus in sarcoma of adult rats inoculated after birth with Rous sarcoma tissue. Nature, London 186:980–981
    [Google Scholar]
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