1887

Abstract

SUMMARY

The DNA molecules synthesized in herpes simplex virus (HSV) infected cells were analysed by electrophoresis in polyacrylamide gels. It was found that part of the virus DNA, isolated from infected nuclei, migrated into the gels in a manner similar to the virus DNA molecules isolated from purified herpes simplex virus particles. Most of the virus DNA molecules, labelled for 60 min with [H]-thymidine as well as labelled cellular DNA molecules, were retained at the top of the gel during electrophoresis. The amount of HSV-DNA which migrated into the gel gradually increased when the labelled cells were reincubated after removal of the isotope (chase). Most of the DNA molecules, which were retained at the top of the gel, had the density of virus DNA but differed in their sedimentation behaviour in sucrose gradients from the intact DNA molecules isolated from purified virus particles. The presence of replicating as well as mature virus DNA molecules in infected nuclei is discussed.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-25-1-63
1974-10-01
2023-02-08
Loading full text...

Full text loading...

/deliver/fulltext/jgv/25/1/JV0250010063.html?itemId=/content/journal/jgv/10.1099/0022-1317-25-1-63&mimeType=html&fmt=ahah

References

  1. Aaij C., Borst P. 1972; The gel electrophoresis of DNA. Biochimica et biophysica acta 269:192–200
    [Google Scholar]
  2. Becker Y., Dym H., Sarov I. 1968; Herpes simplex virus DNA. Virology 36:184–192
    [Google Scholar]
  3. Becker Y., Levitt-Hadar J., Dym H., Olshevsky U. 1971; Effect of the nonionogenic detergent, Nonidet P-40 on enveloped herpes simplex virus. Israel Journal of Medical Sciences 7:656–661
    [Google Scholar]
  4. Becker Y., Neuman S., Levitt-Hadar J. 1972; The effect of the antibiotics distamycin A and congocidine on the replication of herpes simplex virus. Israel Journal of Medical Sciences 8:1225–1230
    [Google Scholar]
  5. Becker Y., Olshevsky U. 1973; Inhibition of herpes simplex virus replication by camptothecin. Israel Journal of Medical Sciences 9:1578–1581
    [Google Scholar]
  6. Dingman C. W., Fisher M. P., Kakefuda T. 1972; Role of molecular conformation in determining the electrophoretic properties of polynucleotides in agarose-acrylamide gels. II. Biochemistry II:1242–1250
    [Google Scholar]
  7. Fischer M. P., Dingman C. W. 1971; Role of molecular conformation in determining the electrophoretic properties of polynucleotides in agarose-acrylamide composite gels. Biochemistry II:1895–1899
    [Google Scholar]
  8. Frenkel N., Roizman B. 1972; Separation of the herpesvirus deoxyribonucleic acid duplex into unique fragments and intact strand and sedimentation in alkaline gradients. Journal of Virology 10:565–572
    [Google Scholar]
  9. Gregson S. 1972; Polyacrylamide gel electrophoresis of DNA. Analytical Biochemistry 48:613–616
    [Google Scholar]
  10. Levitt J., Becker Y. 1967; The effect of cytosine arabinoside on the replication of herpes simplex virus. Virology 31:129–134
    [Google Scholar]
  11. Loening U. E., Ingle J. 1967; Diversity of RNA components in green plant tissues. Nature, London 315:363–367
    [Google Scholar]
  12. Marmur J., Tso P. O. P. 1961; Denaturation of deoxyribonucleic acid by formamide. Biochimica et biophysica acta 51:32–36
    [Google Scholar]
  13. Olshevsky U., Levitt J., Becker Y. 1967; Studies on the synthesis of herpes simplex virions. Virology 33:323–334
    [Google Scholar]
  14. Rosenkranz H. S., Becker Y. 1973; Reversible inhibition of herpes simplex virus replication by hydroxyurea. Antimicrobial Agents and Chemotherapy 3:325–331
    [Google Scholar]
  15. Russell W. C., Gold E., Keir H. M., Omura H., Watson D. H., Wildy P. 1964; The growth of herpes simplex virus and its nucleic acid. Virology 22:103–110
    [Google Scholar]
  16. Sussenbach J. S., Van Der vliet P. C., Ellens D. J., Jansz H. S. 1973; Linear intermediates in the replication of adenovirus DNA. Nature New Biology 239:47–49
    [Google Scholar]
  17. Tegtmeyer P., Masaret F. 1973; Simian virus 40 deoxyribonucleic acid synthesis: analysis by gel electrophoresis. Journal of Virology 10:599–604
    [Google Scholar]
  18. Wilkie N. M. 1973; The synthesis and substructure of herpesvirus DNA: The distribution of alkaline-labile single-strand interruptions in HSV-l DNA. Journal of General Virology 21:453–467
    [Google Scholar]
  19. Williamson R. 1970; The isolation of rapidly labelled deoxyribonucleic acid fragments of nuclear origin from the polyribosome fraction of cultured embryonic line cells. Biochemical Journal 117:59P–60P
    [Google Scholar]
  20. Zeiger R. S., Salomon R., Dingman C. W., Peacock A. C. 1972; Role of base composition in the electrophoresis of microbial and crab DNA in polyacrylamide gels. Nature New Biology 238:65–69
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-25-1-63
Loading
/content/journal/jgv/10.1099/0022-1317-25-1-63
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error