Infectivity of Mengovirus Replicative Form. Relationship to Cellular Transcription Free

Abstract

SUMMARY

Mengovirus-induced, double-stranded RNA (RF) is infective, but its infectivity, unlike that of mengovirus, is strictly dependent upon host cell macromolecular synthesis. The treatment of cells with actinomycin D, -amanitin or cordycepin 1 h before infection with mengovirus RF results in a drastic reduction of virus yield, whereas the same treatment has no effect on mengovirus infectivity. The kinetics of sensitivity to inhibitors suggest that the cellular macromolecule necessary for RF to initiate its infective cycle is involved only during the very early steps of replication, and probably has a very rapid turn-over. The cellular uptake of the infecting molecule seems not to be alterated by actinomycin treatment. Analysis of the intracellular distribution of [H]- or [P]-labelled mengovirus RF indicates that up to 40% of incoming molecules accumulate within the nuclear fraction (4 to 5% in nucleoli). Sedimentation velocity analyses of labelled RF recovered from each subcellular compartment show that the input molecule becomes heavier and polydisperse in gradients as the cycle of infection proceeds. A replication mechanism is proposed in which infective RF is transformed into replicative intermediate (RI), by a cellular RNA polymerase transcribing the first virus messenger RNAs with RF as abnormal template.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-25-1-53
1974-10-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/jgv/25/1/JV0250010053.html?itemId=/content/journal/jgv/10.1099/0022-1317-25-1-53&mimeType=html&fmt=ahah

References

  1. Adesnik M., Salditt M., Thomas W., Darnell J. 1972; Evidence that all messenger RNA molecules (except histone messenger RNA) contains poly (A) sequences and that poly (A) has a nuclear function. Journal of Molecular Biology 71:21–30
    [Google Scholar]
  2. Ascione R., Arlinghaus R. B., Vande Woude G. F. 1972 In Protein Biosynthesis in Non-bacterial Systems chapter 359–116 Edited by Last J. A., Laskin A. I. New York: Marcel Dekker Inc;
    [Google Scholar]
  3. Baltimore D. 1964; In vitro synthesis of viral RNA by the poliovirus RNA-polymerase. Proceedings of the National Academy of Sciences of the United States of America 51:450–456
    [Google Scholar]
  4. Baltimore D. 1969 In Biochemistry of Viruses chapter 3101–176 Edited by Levy H. New York: Marcell Dekker Inc;
    [Google Scholar]
  5. Baltimore D., Eggers H. S., Franklin R. M., Tamm I. 1963; Poliovirus-induced RNA polymerase and the effect of virus-specific inhibitors on its production. Proceedings of the National Academy of Sciences of the United States of America 49:843–849
    [Google Scholar]
  6. Baltimore D., Franklin R. M. 1962; Preliminary date on a virus-specific enzyme system responsible for the synthesis of viral RNA. Biochemical and Biophysical Research Communications 9:388–392
    [Google Scholar]
  7. Baltimore D., Franklin R. M. 1963; A new ribonucleic acid polymerase appearing after mengovirus infection of L-cells. Journal of Biological Chemistry 238:3395–3400
    [Google Scholar]
  8. Baltimore D., Girard M. 1966; An intermediate in the synthesis of poliovirus RNA. Proceedings of the National Academy of Sciences of the United States of America 56:741–748
    [Google Scholar]
  9. Bishop J. M., Koch G. 1967; Purification and characterisation of poliovirus-induced, infectious, double-stranded ribonucleic acid. Journal of Biological Chemistry 242:1736–1743
    [Google Scholar]
  10. Bishop J. M., Koch G. 1969; Infectious replicative intermediate of poliovirus: purification and characterization. Virology 37:521–534
    [Google Scholar]
  11. Chambon P., Gissinger F., Kedinger G., Mandel J. L., Meilnac M., Nuret P. 1972; Structural and functional properties of three mammalian nuclear DNA-dependent RNA polymerases. Karolinska Symposia on Research Methods in Reproductive Endocrinology 5th Symposium 222–246
    [Google Scholar]
  12. Darnbrough G., Hunt T., Jackson R. J. 1972; A complex between met-tRNAt and native 40 S subunits in reticulocyte lysates and its disappearance during incubation with double-stranded RNA. Biochemical and Biophysical Research Communications 48:1556–1564
    [Google Scholar]
  13. Dianzani F., Rita G., Cantagalli P., Gagnoni S. 1969; Effect of DEAE dextran on interferon production and protective effect in mice treated with the double-stranded polynucleotide complex poly inosinic poly cytidylic acid. Journal of Immunology 102:24–27
    [Google Scholar]
  14. Ehrenfeld E., Hunt T. 1971; Double-stranded poliovirus RNA inhibits initiation of protein synthesis by reticulocyte lysate. Proceedings of National Academy of Sciences of the United States of America 68:1075–1078
    [Google Scholar]
  15. Haselkorn R. 1964; Actinomycin D as a probe for nucleic acid secondary structure. Science, New York 143:682–684
    [Google Scholar]
  16. Hastie M. D., ArmStrong S. J., Mahy B. W. J. 1972; Effect of α-amanitin on protein and nucleic acid synthesis in chick embryo fibroblast cells. Biochemical Journal 130:28–29
    [Google Scholar]
  17. Havliza D., Koch G. 1971; Complex formation between poliovirus RNA and polycations. Archives of Biochemistry and Biophysics 147:85–91
    [Google Scholar]
  18. Hunt T., Ehrenfeld E. 1971; Cytoplasm from poliovirus-infected HeLa cells inhibits cell-free haemoglobin synthesis. Nature New Biology 230:91–94
    [Google Scholar]
  19. Koch G., Bishop J. M. 1968; The effect of polycations on the interactions of viral RNA with mammalian cells: studies on the infectivity of single-stranded and double-stranded poliovirus RNA. Virology 35:9–17
    [Google Scholar]
  20. Koch G., Quintrell N., Bishop J. M. 1967; Differential effect of actinomycin D on the infectivity of single- and double-stranded poliovirus RNA. Virology 31:388–390
    [Google Scholar]
  21. Mahy B. W. J., Hastie N. D., Armstrong S. J. 1972; Inhibition of influenza virus replication by α-amanitin: mode of action. Proceedings of the National Academy of Sciences of the United States of America 69:1421–1424
    [Google Scholar]
  22. Mechali M., Perez-Bercoff R., Carrara O., Falcoff E. 1973; An improved methodology for preparation of virus-induced double-stranded RNA. Biochimie 55:361–363
    [Google Scholar]
  23. Montagnier L., Sanders F. K. 1963; Replicative form of encephalomyocarditis virus ribonucleic acid. Nature, London 199:664–667
    [Google Scholar]
  24. Natori S., Takeuchi K., Takahashi K., Mizuno D. 1973; DNA-dependent RNA polymerase from Ehrlich ascites tumor cells. II. Factors affecting the activity of RNA polymerase II. Journal of Biochemistry 73:879–888
    [Google Scholar]
  25. Pagano J. S. 1970; Biological activity of isolated viral nucleic acids. Progress in Medical Virology 12:1–48
    [Google Scholar]
  26. Penman S. 1969 In Fundamental Techniques in Virology chapters 5 and 6 35–57 Edited by Habel W., Salzman M. P. New York and London: Academic Press;
    [Google Scholar]
  27. Pérez-Bercoff R., Carrara G., Dolei A., Conciatori G., Rita G. 1974; In vitro binding of a cellular, α-amanitin sensitive, RNA polymerase to infectious, mengovirus-induced double-stranded RNA. Biochemical and Biophysical Research Communications 56:876–883
    [Google Scholar]
  28. Robertson H. D., Mathews M. B. 1973; Double-stranded RNA as an inhibitor of protein synthesis and as a substrate for a nuclease in extracts of Krebbs II ascites cells. Proceedings of the National Academy of Sciences of the United States of America 70:225–229
    [Google Scholar]
  29. Seifart K. H., Benecke B. J., Juhasz P. P. 1972; Multiple RNA polymerase species from rat liver tissue: possible existence of a cytoplasmic enzyme. Archives of Biochemistry and Biophysics 151:519–532
    [Google Scholar]
  30. Sobell H. M., Jain S., Sakore T. D., Nordman C. E. 1971; Stereochemistry of actinomycin DNA binding. Nature New Biology 231:200–205
    [Google Scholar]
  31. Sudgen B., Keller W. 1973; Mammalian deoxyribonucleic acid-dependent ribonucleic acid polymerase. 1. Purification and properties of an α-amanitin sensitive, ribonucleic acid polymerase and stimulatory factors from HeLa and KB cells. Journal of Biological Chemistry 248:3777–3788
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-25-1-53
Loading
/content/journal/jgv/10.1099/0022-1317-25-1-53
Loading

Data & Media loading...

Most cited Most Cited RSS feed