RT Journal Article SR Electronic(1) A1 Manor, Daphna A1 Barzilai, R. A1 Goldblum, N.YR 1974 T1 Dependence of RNA Replication on Continuous Protein Synthesis in a Temperature-sensitive Mutant of Foot-and-Mouth Disease Virus JF Journal of General Virology, VO 25 IS 1 SP 157 OP 161 DO https://doi.org/10.1099/0022-1317-25-1-157 PB Microbiology Society, SN 1465-2099, AB SUMMARY A temperature-sensitive mutant of foot-and-mouth disease virus (FMDV) of the RNA− phenotype induces active RNA dependent RNA polymerase only at the permissive temperature. Once induced this enzyme is active when assayed under cell-free conditions, even at the non-permissive temperature. However, when the enzyme is induced at the permissive temperature and the cultures are shifted to the non-permissive temperature, a sharp decline in activity is observed. The RNA− character can be phenocopied by blocking protein synthesis. The evidence obtained suggests that virus RNA replication is dependent on continuous production of the enzyme., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-25-1-157