%0 Journal Article %A Manor, Daphna %A Barzilai, R. %A Goldblum, N. %T Dependence of RNA Replication on Continuous Protein Synthesis in a Temperature-sensitive Mutant of Foot-and-Mouth Disease Virus %D 1974 %J Journal of General Virology, %V 25 %N 1 %P 157-161 %@ 1465-2099 %R https://doi.org/10.1099/0022-1317-25-1-157 %I Microbiology Society, %X SUMMARY A temperature-sensitive mutant of foot-and-mouth disease virus (FMDV) of the RNA− phenotype induces active RNA dependent RNA polymerase only at the permissive temperature. Once induced this enzyme is active when assayed under cell-free conditions, even at the non-permissive temperature. However, when the enzyme is induced at the permissive temperature and the cultures are shifted to the non-permissive temperature, a sharp decline in activity is observed. The RNA− character can be phenocopied by blocking protein synthesis. The evidence obtained suggests that virus RNA replication is dependent on continuous production of the enzyme. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-25-1-157