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Dependence of RNA Replication on Continuous Protein Synthesis in a Temperature-sensitive Mutant of Foot-and-Mouth Disease Virus
Abstract
SUMMARY
A temperature-sensitive mutant of foot-and-mouth disease virus (FMDV) of the RNA− phenotype induces active RNA dependent RNA polymerase only at the permissive temperature. Once induced this enzyme is active when assayed under cell-free conditions, even at the non-permissive temperature. However, when the enzyme is induced at the permissive temperature and the cultures are shifted to the non-permissive temperature, a sharp decline in activity is observed. The RNA− character can be phenocopied by blocking protein synthesis. The evidence obtained suggests that virus RNA replication is dependent on continuous production of the enzyme.
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© Journal of General Virology 1974