A temperature-sensitive mutant of foot-and-mouth disease virus (FMDV) of the RNA phenotype induces active RNA dependent RNA polymerase only at the permissive temperature. Once induced this enzyme is active when assayed under cell-free conditions, even at the non-permissive temperature. However, when the enzyme is induced at the permissive temperature and the cultures are shifted to the non-permissive temperature, a sharp decline in activity is observed. The RNA character can be phenocopied by blocking protein synthesis. The evidence obtained suggests that virus RNA replication is dependent on continuous production of the enzyme.


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