1887

Abstract

SUMMARY

The kinetic data obtained from examination of restriction enzyme fragments give a direct determination of the position of the origin of replication in polyoma virus DNA. The results obtained are consistent with the previous estimates based on electron microscopic measurement of replicating molecules cleaved by endonuclease EcoR (29 ± 2% from the EcoR site). The position of the terminus is diametrically opposite the origin, at approx. 79% clockwise from the EcoR site.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-25-1-133
1974-10-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/jgv/25/1/JV0250010133.html?itemId=/content/journal/jgv/10.1099/0022-1317-25-1-133&mimeType=html&fmt=ahah

References

  1. Bourgaux P., Bourgaux-Ramoisy D., Seiler P. 1971; The replication of the ring-shaped DNA of polyoma virus. II. Identification of molecules at various stages of replication. Journal of Molecular Biology 59:195–206
    [Google Scholar]
  2. Crawford L. V., Syrett C., Wilde A. 1973; The replication of polyoma DNA. Journal of General Virology 21:515–521
    [Google Scholar]
  3. Danna K. J., Nathans D. 1972; Bidirectional replication of simian virus 40 DNA. Proceedings of the National Academy of Sciences of the United States of America 69:3097–3100
    [Google Scholar]
  4. Dintzis H. M. 1961; Assembly of the peptide chains of hemoglobin. Proceedings of the National Academy of Sciences of the United States of America 47:247–261
    [Google Scholar]
  5. Fareed G. C., Garon C. F., Salzman N. P. 1972; Origin and direction of simian virus 40 deoxyribonucleic acid replication. Journal of Virology 10:484–491
    [Google Scholar]
  6. Follett E. A. C., Crawford L. V. 1968; Electron microscope study of the denaturation of polyoma virus DNA. Journal of Molecular Biology 34:565–573
    [Google Scholar]
  7. Galibert F., Sedat J., Ziff E. 1974; Direct determination of DNA nucleotide sequences: Structure of a fragment of bacteriophage 96×174 DNA. Journal of Molecular Biology (in the press)
    [Google Scholar]
  8. Griffin B., Fried M., Cowie A. 1974; Polyoma DNA-a physical map. Proceedings of the National Academy of Sciences of the United States of America 71:2077–2081
    [Google Scholar]
  9. Gromkova R., Goodgal S. H. 1972; Action of hemophilus endodeoxyribonuelease on biologically active deoxyribonucleic acid. Journal of Bacteriology 109:987–992
    [Google Scholar]
  10. Hirt B. 1967; Selective extraction of polyoma DNA from infected mouse cell cultures. Journal of Molecular Biology 26:365–369
    [Google Scholar]
  11. Levine A. J., Kang H. S., Billheimer F. E. 1970; DNA replication in SV40 infected cells. 1. Analysis of replicating SV40 DNA. Journal of Molecular Biology 50:549–568
    [Google Scholar]
  12. Ling V. 1972; Fractionation and sequences of the large pyrimidine oligonucleotides from bacteriophage fd DNA. Journal of Molecular Biology 64:87–102
    [Google Scholar]
  13. Middleton J. H., Edgell M. H., Hutchison C. A. H. 1972; Specific fragments of 4×174 deoxyribonucleic acid produced by a restriction enzyme from Haemophilus aegyptius, Endonuclease Z. Journal of Virology 10:42–50
    [Google Scholar]
  14. Nathans D., Danna K. J. 1972; Specific origin in SV40 DNA replication. Nature New Biology 236:200–202
    [Google Scholar]
  15. Radloff F., Bauer W., Vinograd I. 1967; A dye-buoyant-density method for the detection and isolation of closed circular duplex DNA: The closed circular DNA in HeLa cells. Proceedings of the National Academy of Sciences of the United States of America 57:1514–1521
    [Google Scholar]
  16. Sharp P. A., Sugden B., Sambrook J. 1973; Detection of two restriction endonuclease activities in H. parainfluenzae using analytical agarose-ethidium bromide electrophoresis. Biochemistry 12:3055–3063
    [Google Scholar]
  17. Yoshimori R. N. 1971; A genetic and biochemical analysis of the restriction and modification of DNA by resistance transfer factors. Ph. D. thesis University of California; San Francisco Medical Center:
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-25-1-133
Loading
/content/journal/jgv/10.1099/0022-1317-25-1-133
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error