1887

Abstract

SUMMARY

Semliki Forest virus (SFV) established a persistent, non-cytopathic infection in cultured cells with no effect on cumulative cell number as compared with control cultures. All cells were initially infected by SFV as judged by infective centre and immunofluorescence assay and released approx. 50 to 70 p.f.u./cell in the initial 24 h after infection. At 12 h after infection there was a ‘shut-down’ of virus-specific RNA synthesis followed by a sharp decline in the percentage of cells infected, stabilizing at about 2% by 48 h. Significant amounts of virus were released from about 2% of cells in persistently infected cultures. At 8 to 10 days a small-plaque, attenuated variant of SFV appeared in infected cultures; it replaced ‘wild-type’ virus completely by day 16.

The establishment of persistence could not be related to (i) the release of interferon-like activity from infected cells, (ii) the presence of a directly antiviral agent in infected cells or medium, or (iii) changes in the total levels or subcellular distribution of acid phosphatase.

Subculturing persistently infected cells stimulated cell division and promoted a (reduced) burst of virus production; simultaneous superinfection with SFV gave no increase in virus yields.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-24-3-453
1974-09-01
2021-10-19
Loading full text...

Full text loading...

/deliver/fulltext/jgv/24/3/JV0240030453.html?itemId=/content/journal/jgv/10.1099/0022-1317-24-3-453&mimeType=html&fmt=ahah

References

  1. Buckley S. M. 1969; Susceptibility of the Aedes albopictus and Aedes aegypti cell lines to infection with arboviruses. Proceedings of the Society for Experimental Biology and Medicine 131:625–630
    [Google Scholar]
  2. Buckley S. M. 1971; Multiplication of Chikungunya and O’nyong-nyong viruses in Singh’s Aedes cell lines. In Current Topics in Microbiology and Immunology vol 55133–137 Edited By Weiss E. New York: Springer Verlag;
    [Google Scholar]
  3. Dalgarno L., Davey M. W. 1973; Virus replication. In Viruses and Invertebrates Frontiers of Biology, vol 31 chapter 14 245–270 Edited By Gibbs A. J. Amsterdam: North Holland:
    [Google Scholar]
  4. Davey M. W., Dennett D. P., Dalgarno I. 1973; The growth of two togaviruses in cultured mosquito and vertebrate cells. Journal of General Virology 20:225–232
    [Google Scholar]
  5. Enzmann P.-J. 1973; Induction of an interferon-like substance in persistently infected Aedes albopictus cells. Archiv fur die gesamte Virusforschung 41:382–389
    [Google Scholar]
  6. Grace T. D. C. 1962; Establishment of four strains of cells, from insect tissues grown in vitro. Nature, London 195:788–789
    [Google Scholar]
  7. Linhardt K., Walter K. 1965; Phosphatases (phosphomonoesterases). In Methods of Enzymatic Analysis (2nd printing) 779–785 Edited by Bergmeyer H.-U. New York: Academic Press;
    [Google Scholar]
  8. Peleg J. 1969; Inapparent persistent virus infection in continuously grown Aedes aegypti mosquito cells. Journal of General Virology 5:463–471
    [Google Scholar]
  9. Peleg J. 1971; Attenuation of Semliki Forest (SF) virus in continuously cultured Aedes aegypti mosquito cells (Peleg) as a step in the production of vaccines. In Current Topics in Microbiology and Immunology vol 55155–161 Edited by Weiss E. New York: Springer-Verlag:
    [Google Scholar]
  10. Peleg J. 1972; Studies on the behaviour of arboviruses in an Aedes aegypti mosquito cell line (Peleg). Archiv fur die gesamte Virusforschung 37:54–61
    [Google Scholar]
  11. Raghow R. S., Davey M. W., Dalgarno L. 1973a; The growth of Semliki Forest virus in cultured mosquito cells: ultrastructural observations. Archiv fur die gesamte Virusforschung 43:165–168
    [Google Scholar]
  12. Raghow R. S., Grace T. D. C., Filshie B. K., Bartley W., Dalgarno L. 1973b; Ross River virus replication in cultured mosquito and mammalian cells: virus growth and correlated ultrastructural changes. Journal of General Virology 21:109–122 (1973)
    [Google Scholar]
  13. Rehacek I. 1968; The growth of arboviruses in mosquito cells in vitro. Acta virologica 12:241–246
    [Google Scholar]
  14. Shenk T. E., Koshelnyk K. A., Stollar V. 1974; Temperature-sensitive virus from Aedes albopictus cells chronically infected with Sindbis virus. Journal of Virology 13:439–447
    [Google Scholar]
  15. Shine J., Dalgarno L. 1973; Occurrence of heat-dissociable ribosomal RNA in insects: the presence of three polynucleotide chains in 26 S RNA from cultured Aedes aegypti cells. Journal of Molecular Biology 75:57–72
    [Google Scholar]
  16. Sinarachatanant P., Olson L. C. 1973; Replication of Dengue virus type 2 in Aedes albopictus cell culture. Journal of Virology 12:275–283
    [Google Scholar]
  17. Singh K. R. P. 1967; Cell cultures from larvae of Aedes albopictus (Skuse) and Aedes aegypti (L.). Current Science 36:506–508
    [Google Scholar]
  18. Singh K. R. P. 1971; Growth or arboviruses in Aedes albopictus and Aedes aegypti cell lines. In Current Topics in Microbiology and Immunology vol 55127–133 Edited by Weiss E. New York: Springer-Verlag;
    [Google Scholar]
  19. Stollar V., Shenk T. E. 1973; Homologous viral interference in Aedes albopictus cultures chronically infected with Sindbis virus. Journal of Virology 11:592–595
    [Google Scholar]
  20. Walker D. L. 1964; The viral carrier state in animal cell cultures. In Progress in Medical Virology vol 6111–148 Edited by Melnick J. L. Basel: S. Karger;
    [Google Scholar]
  21. Walker D. L., Hinze H. C. 1962; A carrier state of mumps virus in human conjunctiva cells. I. General characteristics. Journal of Experimental Medicine 116:739–750
    [Google Scholar]
  22. Yunker C. E. 1971; Arthropod tissue culture in the study of Arboviruses and Rickettsiae: a review. In Current Topics in Microbiology and Immunology vol 55:113–126 Edited by Weiss E. New York: Springer-Verlag;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-24-3-453
Loading
/content/journal/jgv/10.1099/0022-1317-24-3-453
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error