The early stages of infection of Chenopodium amaranticolor leaves with two temperature-sensitive mutants of tobacco rattle virus and the wild-type strain from which they were derived were investigated by following the development of resistance of potential lesions to inactivation by u.v. light. Resistance began to increase between and 3 h after inoculation under permissive conditions (wild-type strain at 20 °C or 30 °C, mutants at 20 °C), but failed to occur with the mutants under restrictive conditions (30 °C). These results imply that at 30 °C the RNA of the mutants fails to replicate in the initially infected cells.
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