Abortive transformation of human embryonic fibroblasts was induced by Molluscum contagiosum virus. The parameters studied in order to test the establishment of a persistent infection and transformation of cells were: (1) virus interference and interferon production; (2) alterations of cell morphology and growth; (3) presence of neoantigens; (4) karyotype. The possible mechanisms leading to an abortive transformation are discussed.
DourmashkinR.,
FebvreH. L.1958; Culture in vitro sur des cellules de la souche HeLa et identification au microscope electronique du virus du MoUuscum contagiosum. Compte rendu de I’Academie des sciences 346:2308–2310
Friedman-KienA. E.,
VilcekJ.1967; Induction of interference and interferon synthesis by non-replicating Molluscum contagiosum virus. Journal of Immunology 99:1092–1098
JoklikW. K.,
WoodroofeG. M.,
HolmesI. H.,
FennerF.1960; The reactivation of Poxviruses. I. Demonstration of the phenomenon and techniques of assay. Virology n:168–184
KoziorowskaJ.,
WlodarskiK.,
MazurowaN.1971; Transformation of mouse embryo cells by vaccinia virus. Journal of the National Cancer Institute 46:225–241
La PlacaM.1966; On the mechanism of the cytopathic changes produced in human amnion cell cultures by the Molluscum contagiosum virus. Archiv fiir die gesamte Virusforschung 18:374–378
La PlacaM.,
PortolaniM.,
Mannini-PalenzonaA.,
Barbanti-BrodanoG.,
BernardiniA.1967a; Further studies on the mechanism of the cytopathic changes produced by the Molluscum contagiosum virus into human amnion cell cultures. Giornale di Microbiologia 15:205–216
La PlacaM.,
PortolaniM.,
RosaA.1967b; Lack of reactivating activity in the Molluscum contagiosum virus. Archiv fiir die gesamte Virusforschung 20:251–255
MӧllerG.1961; Demonstration of mouse isoantigens at the cellular level by the fluorescent antibody technique. Journal of Experimental Medicine 114:415–434
NevaF. A.1962; Studies on Molluscum contagiosum. Observations on the cytopathic effect of Molluscum suspensions in vitro. Archives of Internal Medicine no:720–725
PalmJ.,
HeynerS.,
BrinsterR. L.1971; Differential immunofluorescence of fertilized mouse eggs with H-2 and non-H-2 antibody. Journal of Experimental Medicine 133:1282–1293
PirieG. D.,
BishopP. M.,
BurkeD. C.,
PostlethwaiteR.1971; Some properties of purified Molluscum contagiosum virus. Journal of General Virology 13:311–320
RoosaR. A.,
BaileyE.1970; DNA-mediated transformation of mammalian cells in culture: increased transforming efficiency following sonication. Journal of Cellular Physiology 75:137–150