%0 Journal Article %A Monner, D. A. %A Boman, H. G. %T Host-dependent Properties of Coliphage ϕW and its Female-specific Host-range Mutants ϕ3 and ϕ4 %D 1974 %J Journal of General Virology, %V 24 %N 1 %P 67-75 %@ 1465-2099 %R https://doi.org/10.1099/0022-1317-24-1-67 %I Microbiology Society, %X SUMMARY Coliphage ϕW and its female-specific host range mutants φ3 and ϕ4 were grown on Escherichia coli B and strain D31, an LPS mutant of E. coli K12. Comparison of phage samples from centre and halo of single plaques indicates that host-range mutants of ϕ3 type are enriched in halos on strain D31. For ϕW there was a decreased adsorption to full-grown cells, for ϕ3 there was only a minor change. Caesium chloride gradients have shown that ϕ3 is denser than ϕW and ϕ4. After growth on strain D31 both ϕW and ϕ3 produced non-infectious tail-less particles, and burst sizes were reduced correspondingly. It is suggested that the assembly of the tail could be blocked by an assumed LPS precursor. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-24-1-67