1887

Abstract

SUMMARY

Active herpes simplex virus-specific thymidine kinase was precipitated with specific antiserum from extracts of infected BHK 21 cells which had been differently labelled with radioactive amino acids before and after infection. Analysis of the content of pre- and post-infection isotope in polypeptides separated electrophoretically from these immune-precipitates demonstrated the single, virus-specific, polypeptide labelled with post-infection label. No new polypeptide was detected with the pre-infection label and the low content of pre-infection isotope indicated the absence of any pre-formed host protein in the herpes thymidine kinase. The thymidine kinase specific antiserum, raised in rabbits against infected rabbit kidney cells, precipitated polypeptides of indistinguishable mol. wt. (44000) from BHK 21 cells and HEp-2 cells infected with the same strain of herpes simplex type 1.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-24-1-215
1974-07-01
2024-12-08
Loading full text...

Full text loading...

/deliver/fulltext/jgv/24/1/JV0240010215.html?itemId=/content/journal/jgv/10.1099/0022-1317-24-1-215&mimeType=html&fmt=ahah

References

  1. Buchan A., Luff S., Wallis C. 1970; Failure to demonstrate the interaction of subunits of thymidine kinase in cells simultaneously infected with herpesvirus and kinaseless mutant. Journal of General Virology 9:239–242
    [Google Scholar]
  2. Buchan A., Watson D. H. 1969; The immunological specificity of thymidine kinase in cells infected with viruses of the herpes group. Journal of General Virology 4:461–463
    [Google Scholar]
  3. Dimmock N. J., Watson D. H. 1969; Proteins specified by influenza virus in infected cells: Analysis by polyacrylamide gell electrophoresis of antigens not present in the virus particle. Journal of General Virology 5:499–509
    [Google Scholar]
  4. Dubbs D. R., Kit S. 1964; Mutant strains of herpes simplex virus deficient in thymidine kinase inducing activity. Journal of General Virology 22:493–502
    [Google Scholar]
  5. Hay J., Perera P. A. J., Morrison J. M., Gentry G. A., Subak-Sharpe J. H. 1971 In Strategy of the VirUS Genome (CIBA Foundation Symposium) Edited by Wolstenholme G. E. W., O’Connor M. Churchill: Livingstone;
    [Google Scholar]
  6. Honess R. W., Watson D. H. 1974; Herpes simplex virus-specific polypeptides studied by polyacrylamide gel electrophoresis of immune precipitates. Journal of General Virology 22:171–185
    [Google Scholar]
  7. Klemperer H. G., Haynes G. R., Shedden W. I. H., Watson D. H. 1967; A virus specific thymidine kinase in BHK-21 cells infected with herpes simplex virus. Virology 31:120–128
    [Google Scholar]
  8. Robinson D. J., Watson D.H. 1971; Structural proteins of herpes simplex virus. Journal of General Virology 10:163–171
    [Google Scholar]
  9. Sim C., Watson D. H. 1973; The role of type specific and cross reacting structural antigens in the neutralization of herpes simplex virus types 1 and 2. Journal of General Virology 19:217–233
    [Google Scholar]
  10. Thouless M. E., Skinner G. R. B. 1971; Differences in the properties of thymidine kinase produced by cells infected with type 1 and type 2 herpes virus. Journal of General Virology 12:195–197
    [Google Scholar]
  11. Watson D. H. 1969; The separation of herpes virus specific antigens by polyacrylamide gel electrophoresis. Journal of General Virology 4:151–161
    [Google Scholar]
  12. Watson D. H., Shedden W. I. H., Elliott A., Tetsuka T., Wildy P., Bourgaux-Ramoisy D., Gold E. 1966; Virus specific antigens in mammalian cells infected with herpes simplex virus. Immunology 11:399–408
    [Google Scholar]
  13. Watson D. H., Wildy P. 1969; The preparation of ‘monoprecipitin’ antisera to herpes virus specific antigens. Journal of General Virology 4:163–168
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-24-1-215
Loading
/content/journal/jgv/10.1099/0022-1317-24-1-215
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error