The virus-induced polypeptides synthesized in human embryonic lung cells infected with 4, a DNA negative temperature-sensitive mutant of herpes simplex virus type I, were examined at the permissive (34 °C) and non-permissive (39 °C) temperatures using SDS-polyacrylamide gel electrophoresis. Cells infected with 4 at 34 °C synthesized virus-specific polypeptides in the same proportion as did wild-type-infected cells at 34 and 39 °C. Cells infected with 4 at 39 °C exhibited multiple polypeptide defects, the most prominent of which was the inhibition of the synthesis of the major virus capsid polypeptide, VP154. The expression of the temperature-sensitive defect relating to VP154 only occurred prior to 4 h after infection, as shown by shift-up studies. The expression of the temperature-sensitive defect at 39 °C relating to VP154 could be reversed by shifting down infected cells to 34 °C, even after 12 h at the non-permissive temperature. This reversal occurred in the presence of cytosine arabinoside but not in the presence of actinomycin D.


Article metrics loading...

Loading full text...

Full text loading...


Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error